TY - JOUR
T1 - Substantial sex-dependent differences in the response of human scalp hair follicles to estrogen stimulation in vitro advocate gender-tailored management of female versus male pattern balding.
AU - Conrad, Franziska
AU - Ohnemus, Ulrich
AU - Bodo, Enikö
AU - Biro, Tamas
AU - Tychsen, Birte
AU - Gerstmayer, Bernhard
AU - Bosio, Andreas
AU - Schmidt-Rose, Thomas
AU - Altgilbers, Silke
AU - Bettermann, Albrecht
AU - Saathoff, Matthias
AU - Meyer, Wilfried
AU - Paus, Ralf
N1 - Funding Information:
This study was supported in part by a grant from BMBF to R. P. The technical assistance of S. Wegerich, G. Pillnitz-Stolze, and B. Mähnss is gratefully acknowledged. We thank Dr M. Warner of the Karolinska Institutet, Stockholm, for kindly providing the antibody ER-β 503.
PY - 2005/12
Y1 - 2005/12
N2 - In this study, it was investigated how estrogens (17-beta-estradiol, E2) affect the estrogen receptor (ER) expression and gene regulation of male versus female human scalp hair follicles in vitro. Anagen VI follicles from frontotemporal scalp skin were microdissected and organ-cultured for up to 9 d in the presence of E2 (1-100 nm). Immunohistochemistry was performed for ERbeta-expression, known to be predominant in human scalp hair follicles, and for TGF-beta2-expression (as negative key hair growth modulator), and E2-responsive genes in organ-cultured human scalp hair follicles (48 h, 10 nM) were explored by cDNA microarray, using a commercial skin focus chip (Memorec, Cologne, Germany). The distribution pattern of ERbeta and TGF-beta2-immunoreactivity differed between male and female hair follicles after 48 h culture. Of 1300 genes tested, several genes were regulated sex-dependent differently. The study reveals substantial sex-dependent differences in the response of frontotemporal human scalp hair follicles to E2. Recognition and systematic dissection of the E2-dependent gene regulation will be crucial for the development of more effective, gender-tailored management strategies for female versus male pattern balding.
AB - In this study, it was investigated how estrogens (17-beta-estradiol, E2) affect the estrogen receptor (ER) expression and gene regulation of male versus female human scalp hair follicles in vitro. Anagen VI follicles from frontotemporal scalp skin were microdissected and organ-cultured for up to 9 d in the presence of E2 (1-100 nm). Immunohistochemistry was performed for ERbeta-expression, known to be predominant in human scalp hair follicles, and for TGF-beta2-expression (as negative key hair growth modulator), and E2-responsive genes in organ-cultured human scalp hair follicles (48 h, 10 nM) were explored by cDNA microarray, using a commercial skin focus chip (Memorec, Cologne, Germany). The distribution pattern of ERbeta and TGF-beta2-immunoreactivity differed between male and female hair follicles after 48 h culture. Of 1300 genes tested, several genes were regulated sex-dependent differently. The study reveals substantial sex-dependent differences in the response of frontotemporal human scalp hair follicles to E2. Recognition and systematic dissection of the E2-dependent gene regulation will be crucial for the development of more effective, gender-tailored management strategies for female versus male pattern balding.
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U2 - 10.1111/j.1087-0024.2005.10115.x
DO - 10.1111/j.1087-0024.2005.10115.x
M3 - Article
C2 - 16382674
AN - SCOPUS:33644876705
VL - 10
SP - 243
EP - 246
JO - Journal of Investigative Dermatology Symposium Proceedings
JF - Journal of Investigative Dermatology Symposium Proceedings
SN - 1087-0024
IS - 3
ER -