Substantial sex-dependent differences in the response of human scalp hair follicles to estrogen stimulation in vitro advocate gender-tailored management of female versus male pattern balding.

Franziska Conrad, Ulrich Ohnemus, Enikö Bodo, Tamas Biro, Birte Tychsen, Bernhard Gerstmayer, Andreas Bosio, Thomas Schmidt-Rose, Silke Altgilbers, Albrecht Bettermann, Matthias Saathoff, Wilfried Meyer, Ralf Paus

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In this study, it was investigated how estrogens (17-beta-estradiol, E2) affect the estrogen receptor (ER) expression and gene regulation of male versus female human scalp hair follicles in vitro. Anagen VI follicles from frontotemporal scalp skin were microdissected and organ-cultured for up to 9 d in the presence of E2 (1-100 nm). Immunohistochemistry was performed for ERbeta-expression, known to be predominant in human scalp hair follicles, and for TGF-beta2-expression (as negative key hair growth modulator), and E2-responsive genes in organ-cultured human scalp hair follicles (48 h, 10 nM) were explored by cDNA microarray, using a commercial skin focus chip (Memorec, Cologne, Germany). The distribution pattern of ERbeta and TGF-beta2-immunoreactivity differed between male and female hair follicles after 48 h culture. Of 1300 genes tested, several genes were regulated sex-dependent differently. The study reveals substantial sex-dependent differences in the response of frontotemporal human scalp hair follicles to E2. Recognition and systematic dissection of the E2-dependent gene regulation will be crucial for the development of more effective, gender-tailored management strategies for female versus male pattern balding.

Original languageEnglish (US)
Pages (from-to)243-246
Number of pages4
JournalThe journal of investigative dermatology. Symposium proceedings / the Society for Investigative Dermatology, Inc. [and] European Society for Dermatological Research
Volume10
Issue number3
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

Fingerprint

Hair Follicle
Scalp
Sex Characteristics
Estrogens
Transforming Growth Factor beta2
Estrogen Receptor beta
Genes
Skin
Gene Expression Regulation
Oligonucleotide Array Sequence Analysis
Estrogen Receptors
Hair
Germany
Dissection
Estradiol
Immunohistochemistry
In Vitro Techniques
Growth

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Substantial sex-dependent differences in the response of human scalp hair follicles to estrogen stimulation in vitro advocate gender-tailored management of female versus male pattern balding. / Conrad, Franziska; Ohnemus, Ulrich; Bodo, Enikö; Biro, Tamas; Tychsen, Birte; Gerstmayer, Bernhard; Bosio, Andreas; Schmidt-Rose, Thomas; Altgilbers, Silke; Bettermann, Albrecht; Saathoff, Matthias; Meyer, Wilfried; Paus, Ralf.

In: The journal of investigative dermatology. Symposium proceedings / the Society for Investigative Dermatology, Inc. [and] European Society for Dermatological Research, Vol. 10, No. 3, 01.01.2005, p. 243-246.

Research output: Contribution to journalArticle

Conrad, Franziska ; Ohnemus, Ulrich ; Bodo, Enikö ; Biro, Tamas ; Tychsen, Birte ; Gerstmayer, Bernhard ; Bosio, Andreas ; Schmidt-Rose, Thomas ; Altgilbers, Silke ; Bettermann, Albrecht ; Saathoff, Matthias ; Meyer, Wilfried ; Paus, Ralf. / Substantial sex-dependent differences in the response of human scalp hair follicles to estrogen stimulation in vitro advocate gender-tailored management of female versus male pattern balding. In: The journal of investigative dermatology. Symposium proceedings / the Society for Investigative Dermatology, Inc. [and] European Society for Dermatological Research. 2005 ; Vol. 10, No. 3. pp. 243-246.
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