Abstract
In this study, it was investigated how estrogens (17-beta-estradiol, E2) affect the estrogen receptor (ER) expression and gene regulation of male versus female human scalp hair follicles in vitro. Anagen VI follicles from frontotemporal scalp skin were microdissected and organ-cultured for up to 9 d in the presence of E2 (1-100 nm). Immunohistochemistry was performed for ERbeta-expression, known to be predominant in human scalp hair follicles, and for TGF-beta2-expression (as negative key hair growth modulator), and E2-responsive genes in organ-cultured human scalp hair follicles (48 h, 10 nM) were explored by cDNA microarray, using a commercial skin focus chip (Memorec, Cologne, Germany). The distribution pattern of ERbeta and TGF-beta2-immunoreactivity differed between male and female hair follicles after 48 h culture. Of 1300 genes tested, several genes were regulated sex-dependent differently. The study reveals substantial sex-dependent differences in the response of frontotemporal human scalp hair follicles to E2. Recognition and systematic dissection of the E2-dependent gene regulation will be crucial for the development of more effective, gender-tailored management strategies for female versus male pattern balding.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 243-246 |
| Number of pages | 4 |
| Journal | The journal of investigative dermatology. Symposium proceedings / the Society for Investigative Dermatology, Inc. [and] European Society for Dermatological Research |
| Volume | 10 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jan 1 2005 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biotechnology
- Molecular Biology
- Dermatology
- Cell Biology
Cite this
Substantial sex-dependent differences in the response of human scalp hair follicles to estrogen stimulation in vitro advocate gender-tailored management of female versus male pattern balding. / Conrad, Franziska; Ohnemus, Ulrich; Bodo, Enikö; Biro, Tamas; Tychsen, Birte; Gerstmayer, Bernhard; Bosio, Andreas; Schmidt-Rose, Thomas; Altgilbers, Silke; Bettermann, Albrecht; Saathoff, Matthias; Meyer, Wilfried; Paus, Ralf.
In: The journal of investigative dermatology. Symposium proceedings / the Society for Investigative Dermatology, Inc. [and] European Society for Dermatological Research, Vol. 10, No. 3, 01.01.2005, p. 243-246.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Substantial sex-dependent differences in the response of human scalp hair follicles to estrogen stimulation in vitro advocate gender-tailored management of female versus male pattern balding.
AU - Conrad, Franziska
AU - Ohnemus, Ulrich
AU - Bodo, Enikö
AU - Biro, Tamas
AU - Tychsen, Birte
AU - Gerstmayer, Bernhard
AU - Bosio, Andreas
AU - Schmidt-Rose, Thomas
AU - Altgilbers, Silke
AU - Bettermann, Albrecht
AU - Saathoff, Matthias
AU - Meyer, Wilfried
AU - Paus, Ralf
PY - 2005/1/1
Y1 - 2005/1/1
N2 - In this study, it was investigated how estrogens (17-beta-estradiol, E2) affect the estrogen receptor (ER) expression and gene regulation of male versus female human scalp hair follicles in vitro. Anagen VI follicles from frontotemporal scalp skin were microdissected and organ-cultured for up to 9 d in the presence of E2 (1-100 nm). Immunohistochemistry was performed for ERbeta-expression, known to be predominant in human scalp hair follicles, and for TGF-beta2-expression (as negative key hair growth modulator), and E2-responsive genes in organ-cultured human scalp hair follicles (48 h, 10 nM) were explored by cDNA microarray, using a commercial skin focus chip (Memorec, Cologne, Germany). The distribution pattern of ERbeta and TGF-beta2-immunoreactivity differed between male and female hair follicles after 48 h culture. Of 1300 genes tested, several genes were regulated sex-dependent differently. The study reveals substantial sex-dependent differences in the response of frontotemporal human scalp hair follicles to E2. Recognition and systematic dissection of the E2-dependent gene regulation will be crucial for the development of more effective, gender-tailored management strategies for female versus male pattern balding.
AB - In this study, it was investigated how estrogens (17-beta-estradiol, E2) affect the estrogen receptor (ER) expression and gene regulation of male versus female human scalp hair follicles in vitro. Anagen VI follicles from frontotemporal scalp skin were microdissected and organ-cultured for up to 9 d in the presence of E2 (1-100 nm). Immunohistochemistry was performed for ERbeta-expression, known to be predominant in human scalp hair follicles, and for TGF-beta2-expression (as negative key hair growth modulator), and E2-responsive genes in organ-cultured human scalp hair follicles (48 h, 10 nM) were explored by cDNA microarray, using a commercial skin focus chip (Memorec, Cologne, Germany). The distribution pattern of ERbeta and TGF-beta2-immunoreactivity differed between male and female hair follicles after 48 h culture. Of 1300 genes tested, several genes were regulated sex-dependent differently. The study reveals substantial sex-dependent differences in the response of frontotemporal human scalp hair follicles to E2. Recognition and systematic dissection of the E2-dependent gene regulation will be crucial for the development of more effective, gender-tailored management strategies for female versus male pattern balding.
UR - http://www.scopus.com/inward/record.url?scp=33644876705&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33644876705&partnerID=8YFLogxK
U2 - 10.1111/j.1087-0024.2005.10115.x
DO - 10.1111/j.1087-0024.2005.10115.x
M3 - Article
C2 - 16382674
AN - SCOPUS:33644876705
VL - 10
SP - 243
EP - 246
JO - Journal of Investigative Dermatology Symposium Proceedings
JF - Journal of Investigative Dermatology Symposium Proceedings
SN - 1087-0024
IS - 3
ER -