Subsets of Activated CD4 T Lymphocytes Refractory for Secretion of IL-2 Are Distinguished by Expression of Ly-6A/E in BALB/c Mice

Elaine K. Codias, Thomas Malek

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3 Citations (Scopus)

Abstract

Ly-6A/E, a cell surface glycosylphosphatidylinositol-anchored protein that modulates T cell activation, is expressed on developing and mature T cells and is tightly regulated in a haplotype- and subset-specific manner. We examined whether Ly-6A/ElowCD4+ and Ly-6A/EhighCD4+ T cells comprised functional subsets. Peripheral CD4+ T cells were primed in vitro with Con A in the presence or absence of IL-4 or IFN-yγ, sorted for Ly-6A/E expression, and restimulated to induce lymphokine production. Regardless of priming conditions, IL-2 production by Ly-6A/EhighCD4+ effector T cells was markedly reduced (mean = 83%) compared to the Ly-6A/ElowCD4+ subset. The Ly-6A/EhighCD4+ subset also produced less IFN-γ than Ly-6A/ElowCD4+ cells and little IL-4 when primed without exogenous IL-4. In contrast, Ly-6A/EhighCD4+ cells primed with exogenous IL-4 produced ample IL-4 and IFN-γ. Interestingly, the difference in IL-2 production between Ly-6A/Elow and Ly-6A/EhighCD4+ subsets was not due to a failure of the Ly-6A/ElowCD4+ cells to become primed because substantially greater amounts of IL-2 and IL-4 were produced by the Con A-pretreated Ly-6A/ElowCD4+ subset in comparison to unprimed Ly-6A/ElowCD4+ cells. Taken together these data suggest Ly-6A/E marks a subset of CD4+ effector T cells that differs from Ly-6A/ElowCD4+ cells by a greatly reduced capacity to produce IL-2 but not IL-4.

Original languageEnglish
Pages (from-to)5918-5929
Number of pages12
JournalJournal of Immunology
Volume151
Issue number11
StatePublished - Dec 1 1993

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Interleukin-4
Interleukin-2
T-Lymphocytes
Glycosylphosphatidylinositols
Lymphokines
Haplotypes
Proteins

ASJC Scopus subject areas

  • Immunology

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Subsets of Activated CD4 T Lymphocytes Refractory for Secretion of IL-2 Are Distinguished by Expression of Ly-6A/E in BALB/c Mice. / Codias, Elaine K.; Malek, Thomas.

In: Journal of Immunology, Vol. 151, No. 11, 01.12.1993, p. 5918-5929.

Research output: Contribution to journalArticle

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abstract = "Ly-6A/E, a cell surface glycosylphosphatidylinositol-anchored protein that modulates T cell activation, is expressed on developing and mature T cells and is tightly regulated in a haplotype- and subset-specific manner. We examined whether Ly-6A/ElowCD4+ and Ly-6A/EhighCD4+ T cells comprised functional subsets. Peripheral CD4+ T cells were primed in vitro with Con A in the presence or absence of IL-4 or IFN-yγ, sorted for Ly-6A/E expression, and restimulated to induce lymphokine production. Regardless of priming conditions, IL-2 production by Ly-6A/EhighCD4+ effector T cells was markedly reduced (mean = 83{\%}) compared to the Ly-6A/ElowCD4+ subset. The Ly-6A/EhighCD4+ subset also produced less IFN-γ than Ly-6A/ElowCD4+ cells and little IL-4 when primed without exogenous IL-4. In contrast, Ly-6A/EhighCD4+ cells primed with exogenous IL-4 produced ample IL-4 and IFN-γ. Interestingly, the difference in IL-2 production between Ly-6A/Elow and Ly-6A/EhighCD4+ subsets was not due to a failure of the Ly-6A/ElowCD4+ cells to become primed because substantially greater amounts of IL-2 and IL-4 were produced by the Con A-pretreated Ly-6A/ElowCD4+ subset in comparison to unprimed Ly-6A/ElowCD4+ cells. Taken together these data suggest Ly-6A/E marks a subset of CD4+ effector T cells that differs from Ly-6A/ElowCD4+ cells by a greatly reduced capacity to produce IL-2 but not IL-4.",
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