Subconjunctival carboplatin in retinoblastoma: Impact of tumor burden and dose schedule

Brandy H. Hayden, Timothy G. Murray, Ingrid U. Scott, Nicole Cicciarelli, Eleut Hernandez, William Feuer, Lilia Fulton, Joan M. O'Brien

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Objective: To evaluate the impact of tumor burden and chemotherapy dose scheduling on the response to subconjunctival carboplatin treatment in a murine transgenic retinoblastoma model. Methods: Eighty simian virus 40 T antigen-positive mice were treated at age 5 or 10 weeks. Six control animals received placebo treatment. Twenty-four 5-week-old mice received 6 subconjunctival carboplatin injections at doses of 30 to 300 μg delivered at 72-hour intervals. Fifty 10-week-old mice received either 6 or 12 subconjunctival carboplatin injections at doses of 30 to 300 μg delivered at 72-hour intervals. All eyes were obtained at age 16 weeks for histopathologic examination. Eyes were graded as positive if any tumor was present. Results: All simian virus 40 T antigen-positive control eyes contained large tumor foci throughout the retina. Subconjunctival carboplatin injections controlled tumors in a dose-dependent manner. Tumor control was observed in 50% of treated eyes at 138.3 μg for the 10-week-old 6-injection group, 94.3 μg for the 5-week-old 6-injection group, and 85.9 μg for the 10-week-old 12-injection group. Conclusion: Increased tumor burden requires an increase in subconjunctival carboplatin dose scheduling to maintain local tumor control in this murine model of retinoblastoma. Clinical Relevance: This study documents the efficacy of subconjunctival carboplatin in the treatment of an animal model of retinoblastoma. These data establish a framework for further human clinical trials.

Original languageEnglish (US)
Pages (from-to)1549-1554
Number of pages6
JournalArchives of ophthalmology
Volume118
Issue number11
DOIs
StatePublished - Jan 1 2000

ASJC Scopus subject areas

  • Ophthalmology

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    Hayden, B. H., Murray, T. G., Scott, I. U., Cicciarelli, N., Hernandez, E., Feuer, W., Fulton, L., & O'Brien, J. M. (2000). Subconjunctival carboplatin in retinoblastoma: Impact of tumor burden and dose schedule. Archives of ophthalmology, 118(11), 1549-1554. https://doi.org/10.1001/archopht.118.11.1549