Subcellular localization of tbe tRNA processing enzyme, tRNA nucleotidyltransferase, in Xenopus laevis oocytes and in somatic cells

Aldo Solari, Murray P Deutscher

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Earlier studies of the tRNA processing enzyme, tRNA nucleotidyltransferase. in rat liver cells suggested that the enzyme was absent from nuclei, whereas more recent studies with tRNA transcription-processing systems from Xenous laevis oocytes indicated that the enzyme was present in germinal vesicles of these cells. In order to resolve this apparent discrepancy, the subcellular localization of tRNA nucleotidyltransferase in oocytes was directly determined, and its distribution in somatic cells was re-examined. Oocyte germinal vesicles, isolated manually, contained about 25% of the total tRNA nucleotidyltransferase activity of the cell, but the enzyme rapidly leaked out of these nuclei during storage. In contrast, purified nuclei isolated from rat or Xenopus liver using a variety of aqueous procedures were devoid of tRNA nucleotidy transferase activity . Since rapidly-isolated, crude nuclear pre- Parations from liver contained substantially more of the enzyme than could be accounted for by cytoplasmic contamination, it appears that the enzyme leaks out of the somatic cell nuclei during the lengthy times required for their purification . The implications of these results for the development of tRNA processing systems from somatic cell nuclei are discussed.

Original languageEnglish
Pages (from-to)4397-4407
Number of pages11
JournalNucleic Acids Research
Volume10
Issue number14
DOIs
StatePublished - Jul 24 1982
Externally publishedYes

Fingerprint

Oocyte
Xenopus laevis
Transfer RNA
Oocytes
Enzymes
Nucleus
Cell
Processing
Liver
Cells
Vesicles
Cell Nucleus
Rats
Transcription
Transferases
Xenopus
Purification
Contamination
Discrepancy
tRNA nucleotidyltransferase

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Subcellular localization of tbe tRNA processing enzyme, tRNA nucleotidyltransferase, in Xenopus laevis oocytes and in somatic cells. / Solari, Aldo; Deutscher, Murray P.

In: Nucleic Acids Research, Vol. 10, No. 14, 24.07.1982, p. 4397-4407.

Research output: Contribution to journalArticle

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