Subcellular localization of regulator of G protein signaling RGS7 complex in neurons and transfected cells

Evangelos Liapis, Simone Sandiford, Qiang Wang, Gabriel Gaidosh, Dario Motti, Konstantin Levay, Vladlen Z Slepak

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The R7 family of regulators of G protein signaling (RGS) is involved in many functions of the nervous system. This family includes RGS6, RGS7, RGS9, and RGS11 gene products and is defined by the presence of the characteristic first found in Disheveled, Egl-10, Pleckstrin (DEP), DEP helical extension (DHEX), GÎÂ3-like, and RGS domains. Herein, we examined the subcellular localization of RGS7, the most broadly expressed R7 member. Our immunofluorescence studies of retinal and dorsal root ganglion neurons showed that RGS7 concentrated at the plasma membrane of cell bodies, in structures resembling lamellipodia or filopodia along the processes, and at the dendritic tips. At the plasma membrane of dorsal root ganglia neurons, RGS7 co-localized with its known binding partners R7 RGS binding protein (R7BP), Gαo, and Gαq. More than 50% of total RGS7-specific immunofluorescence was present in the cytoplasm, primarily within numerous small puncta that did not co-localize with R7BP. No specific RGS7 or R7BP immunoreactivity was detected in the nuclei. In transfected cell lines, ectopic RGS7 had both diffuse cytosolic and punctate localization patterns. RGS7 also localized in centrosomes. Structure-function analysis showed that the punctate localization was mediated by the DEP/DHEX domains, and centrosomal localization was dependent on the DHEX domain. Numerous neuronal functions are regulated by heterotrimeric G proteins. The main finding of our study is that a large pool of Regulator of G protein Signaling RGS7 is localized in cytoplasmic vesicles and the centrosome, where it does not co-localize with its known binding partners R7BP, Gαq, and Gαo. This points to a novel role of RGS7 in neuronal function in addition to its canonical role at the plasma membrane.

Original languageEnglish
Pages (from-to)568-581
Number of pages14
JournalJournal of Neurochemistry
Volume122
Issue number3
DOIs
StatePublished - Aug 1 2012

Fingerprint

RGS Proteins
GTP-Binding Protein Regulators
Neurons
Carrier Proteins
Cell membranes
Centrosome
Pseudopodia
Cell Membrane
Spinal Ganglia
Fluorescent Antibody Technique
Cells
Cytoplasmic Vesicles
Heterotrimeric GTP-Binding Proteins
Neurology
Plasma Cells
GTP-Binding Proteins
Protein Binding
Nervous System
Cytoplasm
Genes

Keywords

  • centrosome
  • G proteins
  • neurons
  • R7BP
  • RGS protein

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Subcellular localization of regulator of G protein signaling RGS7 complex in neurons and transfected cells. / Liapis, Evangelos; Sandiford, Simone; Wang, Qiang; Gaidosh, Gabriel; Motti, Dario; Levay, Konstantin; Slepak, Vladlen Z.

In: Journal of Neurochemistry, Vol. 122, No. 3, 01.08.2012, p. 568-581.

Research output: Contribution to journalArticle

Liapis, Evangelos ; Sandiford, Simone ; Wang, Qiang ; Gaidosh, Gabriel ; Motti, Dario ; Levay, Konstantin ; Slepak, Vladlen Z. / Subcellular localization of regulator of G protein signaling RGS7 complex in neurons and transfected cells. In: Journal of Neurochemistry. 2012 ; Vol. 122, No. 3. pp. 568-581.
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AU - Levay, Konstantin

AU - Slepak, Vladlen Z

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