Subcellular distribution of corticotropin-releasing-factor-like immunoreactivity in rat central nervous system

S. T. Cain; M. J. Owens; C. B. Nemeroff

doi:10.1159/000125848

Subcellular distribution of corticotropin-releasing-factor-like immunoreactivity in rat central nervous system

S. T. Cain, M. J. Owens, C. B. Nemeroff

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Corticotropin-releasing-factor-like immunoreactivity (CRF-LI) was measured in a number of subcellular fractions from rat brain using a highly sensitive and specific radioimmunoassay. CRF-LI was highly enriched in the crude synaptosomal/mitochondrial fraction (P₂) relative to the homogenate, P₁, S₁, and S₂ fractions. Separation of the P₂ fraction into synaptosomal, myelin, and mitochondria-enriched subfractions on a rapid one-step sucrose gradient revealed that CRF-LI was present at higher concentrations in the synaptosomal fraction than in the mitochondrial and myelin fractions. The distribution of CRF-LI paralleled that of synapsin, a synaptic vesicle marker phosphoprotein, but not that of pyruvate dehydrogenase, a mitochondrial phosphoprotein. These results are consistent with a nerve terminal localization of CRF and a potential role for this peptide as a central nervous system neurotransmitter.

Original language	English (US)
Pages (from-to)	36-41
Number of pages	6
Journal	Neuroendocrinology
Volume	54
Issue number	1
DOIs	https://doi.org/10.1159/000125848
State	Published - 1991
Externally published	Yes

Keywords

central nervous system
corticotropin-releasing factor
phosphoprotein
pyruvate dehydrogenase
subcellular distribution
synapsin
synaptosomes

ASJC Scopus subject areas

Endocrinology
Neuroscience(all)

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title = "Subcellular distribution of corticotropin-releasing-factor-like immunoreactivity in rat central nervous system",

abstract = "Corticotropin-releasing-factor-like immunoreactivity (CRF-LI) was measured in a number of subcellular fractions from rat brain using a highly sensitive and specific radioimmunoassay. CRF-LI was highly enriched in the crude synaptosomal/mitochondrial fraction (P2) relative to the homogenate, P1, S1, and S2 fractions. Separation of the P2 fraction into synaptosomal, myelin, and mitochondria-enriched subfractions on a rapid one-step sucrose gradient revealed that CRF-LI was present at higher concentrations in the synaptosomal fraction than in the mitochondrial and myelin fractions. The distribution of CRF-LI paralleled that of synapsin, a synaptic vesicle marker phosphoprotein, but not that of pyruvate dehydrogenase, a mitochondrial phosphoprotein. These results are consistent with a nerve terminal localization of CRF and a potential role for this peptide as a central nervous system neurotransmitter.",

keywords = "central nervous system, corticotropin-releasing factor, phosphoprotein, pyruvate dehydrogenase, subcellular distribution, synapsin, synaptosomes",

author = "Cain, {S. T.} and Owens, {M. J.} and Nemeroff, {C. B.}",

year = "1991",

doi = "10.1159/000125848",

language = "English (US)",

volume = "54",

pages = "36--41",

journal = "Neuroendocrinology",

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AU - Cain, S. T.

AU - Owens, M. J.

AU - Nemeroff, C. B.

PY - 1991

Y1 - 1991

N2 - Corticotropin-releasing-factor-like immunoreactivity (CRF-LI) was measured in a number of subcellular fractions from rat brain using a highly sensitive and specific radioimmunoassay. CRF-LI was highly enriched in the crude synaptosomal/mitochondrial fraction (P2) relative to the homogenate, P1, S1, and S2 fractions. Separation of the P2 fraction into synaptosomal, myelin, and mitochondria-enriched subfractions on a rapid one-step sucrose gradient revealed that CRF-LI was present at higher concentrations in the synaptosomal fraction than in the mitochondrial and myelin fractions. The distribution of CRF-LI paralleled that of synapsin, a synaptic vesicle marker phosphoprotein, but not that of pyruvate dehydrogenase, a mitochondrial phosphoprotein. These results are consistent with a nerve terminal localization of CRF and a potential role for this peptide as a central nervous system neurotransmitter.

AB - Corticotropin-releasing-factor-like immunoreactivity (CRF-LI) was measured in a number of subcellular fractions from rat brain using a highly sensitive and specific radioimmunoassay. CRF-LI was highly enriched in the crude synaptosomal/mitochondrial fraction (P2) relative to the homogenate, P1, S1, and S2 fractions. Separation of the P2 fraction into synaptosomal, myelin, and mitochondria-enriched subfractions on a rapid one-step sucrose gradient revealed that CRF-LI was present at higher concentrations in the synaptosomal fraction than in the mitochondrial and myelin fractions. The distribution of CRF-LI paralleled that of synapsin, a synaptic vesicle marker phosphoprotein, but not that of pyruvate dehydrogenase, a mitochondrial phosphoprotein. These results are consistent with a nerve terminal localization of CRF and a potential role for this peptide as a central nervous system neurotransmitter.

KW - central nervous system

KW - corticotropin-releasing factor

KW - phosphoprotein

KW - pyruvate dehydrogenase

KW - subcellular distribution

KW - synapsin

KW - synaptosomes

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SN - 0028-3835

IS - 1

ER -

Subcellular distribution of corticotropin-releasing-factor-like immunoreactivity in rat central nervous system

Abstract

Keywords

ASJC Scopus subject areas

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