Abstract
Corticotropin-releasing-factor-like immunoreactivity (CRF-LI) was measured in a number of subcellular fractions from rat brain using a highly sensitive and specific radioimmunoassay. CRF-LI was highly enriched in the crude synaptosomal/mitochondrial fraction (P2) relative to the homogenate, P1, S1, and S2 fractions. Separation of the P2 fraction into synaptosomal, myelin, and mitochondria-enriched subfractions on a rapid one-step sucrose gradient revealed that CRF-LI was present at higher concentrations in the synaptosomal fraction than in the mitochondrial and myelin fractions. The distribution of CRF-LI paralleled that of synapsin, a synaptic vesicle marker phosphoprotein, but not that of pyruvate dehydrogenase, a mitochondrial phosphoprotein. These results are consistent with a nerve terminal localization of CRF and a potential role for this peptide as a central nervous system neurotransmitter.
| Original language | English |
|---|---|
| Pages (from-to) | 36-41 |
| Number of pages | 6 |
| Journal | Neuroendocrinology |
| Volume | 54 |
| Issue number | 1 |
| State | Published - Jan 1 1991 |
| Externally published | Yes |
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Keywords
- central nervous system
- corticotropin-releasing factor
- phosphoprotein
- pyruvate dehydrogenase
- subcellular distribution
- synapsin
- synaptosomes
ASJC Scopus subject areas
- Endocrinology
- Neuroscience(all)
Cite this
Subcellular distribution of corticotropin-releasing-factor-like immunoreactivity in rat central nervous system. / Cain, S. T.; Owens, M. J.; Nemeroff, Charles.
In: Neuroendocrinology, Vol. 54, No. 1, 01.01.1991, p. 36-41.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Subcellular distribution of corticotropin-releasing-factor-like immunoreactivity in rat central nervous system
AU - Cain, S. T.
AU - Owens, M. J.
AU - Nemeroff, Charles
PY - 1991/1/1
Y1 - 1991/1/1
N2 - Corticotropin-releasing-factor-like immunoreactivity (CRF-LI) was measured in a number of subcellular fractions from rat brain using a highly sensitive and specific radioimmunoassay. CRF-LI was highly enriched in the crude synaptosomal/mitochondrial fraction (P2) relative to the homogenate, P1, S1, and S2 fractions. Separation of the P2 fraction into synaptosomal, myelin, and mitochondria-enriched subfractions on a rapid one-step sucrose gradient revealed that CRF-LI was present at higher concentrations in the synaptosomal fraction than in the mitochondrial and myelin fractions. The distribution of CRF-LI paralleled that of synapsin, a synaptic vesicle marker phosphoprotein, but not that of pyruvate dehydrogenase, a mitochondrial phosphoprotein. These results are consistent with a nerve terminal localization of CRF and a potential role for this peptide as a central nervous system neurotransmitter.
AB - Corticotropin-releasing-factor-like immunoreactivity (CRF-LI) was measured in a number of subcellular fractions from rat brain using a highly sensitive and specific radioimmunoassay. CRF-LI was highly enriched in the crude synaptosomal/mitochondrial fraction (P2) relative to the homogenate, P1, S1, and S2 fractions. Separation of the P2 fraction into synaptosomal, myelin, and mitochondria-enriched subfractions on a rapid one-step sucrose gradient revealed that CRF-LI was present at higher concentrations in the synaptosomal fraction than in the mitochondrial and myelin fractions. The distribution of CRF-LI paralleled that of synapsin, a synaptic vesicle marker phosphoprotein, but not that of pyruvate dehydrogenase, a mitochondrial phosphoprotein. These results are consistent with a nerve terminal localization of CRF and a potential role for this peptide as a central nervous system neurotransmitter.
KW - central nervous system
KW - corticotropin-releasing factor
KW - phosphoprotein
KW - pyruvate dehydrogenase
KW - subcellular distribution
KW - synapsin
KW - synaptosomes
UR - http://www.scopus.com/inward/record.url?scp=0025763998&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025763998&partnerID=8YFLogxK
M3 - Article
C2 - 1922675
AN - SCOPUS:0025763998
VL - 54
SP - 36
EP - 41
JO - Neuroendocrinology
JF - Neuroendocrinology
SN - 0028-3835
IS - 1
ER -