Subcellular distribution of corticotropin-releasing-factor-like immunoreactivity in rat central nervous system

S. T. Cain, M. J. Owens, C. B. Nemeroff

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Corticotropin-releasing-factor-like immunoreactivity (CRF-LI) was measured in a number of subcellular fractions from rat brain using a highly sensitive and specific radioimmunoassay. CRF-LI was highly enriched in the crude synaptosomal/mitochondrial fraction (P2) relative to the homogenate, P1, S1, and S2 fractions. Separation of the P2 fraction into synaptosomal, myelin, and mitochondria-enriched subfractions on a rapid one-step sucrose gradient revealed that CRF-LI was present at higher concentrations in the synaptosomal fraction than in the mitochondrial and myelin fractions. The distribution of CRF-LI paralleled that of synapsin, a synaptic vesicle marker phosphoprotein, but not that of pyruvate dehydrogenase, a mitochondrial phosphoprotein. These results are consistent with a nerve terminal localization of CRF and a potential role for this peptide as a central nervous system neurotransmitter.

Original languageEnglish (US)
Pages (from-to)36-41
Number of pages6
Issue number1
StatePublished - 1991
Externally publishedYes


  • central nervous system
  • corticotropin-releasing factor
  • phosphoprotein
  • pyruvate dehydrogenase
  • subcellular distribution
  • synapsin
  • synaptosomes

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)


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