Subcellular distribution of CFTR in rat intestine supports a physiologic role for CFTR regulation by vesicle traffic

Nadia A. Ameen, Elly Van Donselaar, George Posthuma, Hugo De Jonge, Gwenn E McLaughlin, Hans J. Geuze, Christopher Marino, Peter J. Peters

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-activated chloride channel critical to intestinal anion secretion. In addition to phosphorylation, vesicle traffic regulates CFTR in some epithelial cells. Studies of cultured intestinal cells are conflicting regarding the role of cAMP-dependent vesicle traffic in regulating chloride transport. Whether CFTR is present in vesicular compartments within chloride secretory cells in the intestine is unknown and the role of cAMP-dependent vesicle insertion in regulating CFTR and intestinal fluid secretion remains unclear. The purpose of this study was to: (1) examine and quantify the subcellular distribution for CFTR in rat intestine, (2) further define the ultrastructure of the previously identified CFTR High Expresser (CHE) cell, and (3) examine the cellular distribution of CFTR following cAMP stimulation in vivo. Using the sensitive techniques of cryoimmunogold electron microscopy we identified CFTR in subapical vesicles and on the apical plasma membrane in crypt, Brunner glands, and CHE cells. cAMP stimulation in rat proximal small intestine produced a fluid secretory response and was associated with an apical redistribution of CFTR, supporting a physiologic role for cAMP-dependent CFTR vesicle insertion in regulating CFTR in the intestine.

Original languageEnglish
Pages (from-to)219-228
Number of pages10
JournalHistochemistry and Cell Biology
Volume114
Issue number3
StatePublished - Oct 23 2000

Fingerprint

cystic fibrosis
Cystic Fibrosis Transmembrane Conductance Regulator
intestines
regulators
traffic
rats
Intestines
Rats
Phosphorylation
Fluids
Cell membranes
Electron microscopy
Negative ions
Intestinal Secretions
secretions
chlorides
cells
stimulation
Chlorides
insertion

Keywords

  • CFTR
  • Intestine
  • Subcellular localization
  • Vesicle traffic

ASJC Scopus subject areas

  • Cell Biology
  • Instrumentation

Cite this

Ameen, N. A., Van Donselaar, E., Posthuma, G., De Jonge, H., McLaughlin, G. E., Geuze, H. J., ... Peters, P. J. (2000). Subcellular distribution of CFTR in rat intestine supports a physiologic role for CFTR regulation by vesicle traffic. Histochemistry and Cell Biology, 114(3), 219-228.

Subcellular distribution of CFTR in rat intestine supports a physiologic role for CFTR regulation by vesicle traffic. / Ameen, Nadia A.; Van Donselaar, Elly; Posthuma, George; De Jonge, Hugo; McLaughlin, Gwenn E; Geuze, Hans J.; Marino, Christopher; Peters, Peter J.

In: Histochemistry and Cell Biology, Vol. 114, No. 3, 23.10.2000, p. 219-228.

Research output: Contribution to journalArticle

Ameen, NA, Van Donselaar, E, Posthuma, G, De Jonge, H, McLaughlin, GE, Geuze, HJ, Marino, C & Peters, PJ 2000, 'Subcellular distribution of CFTR in rat intestine supports a physiologic role for CFTR regulation by vesicle traffic', Histochemistry and Cell Biology, vol. 114, no. 3, pp. 219-228.
Ameen, Nadia A. ; Van Donselaar, Elly ; Posthuma, George ; De Jonge, Hugo ; McLaughlin, Gwenn E ; Geuze, Hans J. ; Marino, Christopher ; Peters, Peter J. / Subcellular distribution of CFTR in rat intestine supports a physiologic role for CFTR regulation by vesicle traffic. In: Histochemistry and Cell Biology. 2000 ; Vol. 114, No. 3. pp. 219-228.
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