TY - JOUR
T1 - Subarachnoid transplant of the human neuronal hNT2.19 serotonergic cell line attenuates behavioral hypersensitivity without affecting motor dysfunction after severe contusive spinal cord injury
AU - Eaton, Mary J.
AU - Widerström-Noga, Eva
AU - Wolfe, Stacey Quintero
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2011
Y1 - 2011
N2 - Transplant of cells which make biologic agents that can modulate the sensory and motor responses after spinal cord injury (SCI) would be useful to treat pain and paralysis. To address this need for clinically useful human cells, a unique neuronal cell line that synthesizes and secretes/releases the neurotransmitter serotonin (5HT) was isolated. Hind paw tactile allodynia and thermal hyperalgesia induced by severe contusive SCI were potently reversed after lumbar subarachnoid transplant of differentiated cells, but had no effect on open fieldmotor scores, stride length, foot rotation, base of support, or gridwalk footfall errors associatedwith the SCI. The sensory effects appeared 1 week after transplant and did not diminish during the 8-week course of the experiment when grafts were placed 2 weeks after SCI. Many grafted cells were still present and synthesizing 5HT at the end of the study. These data suggest that the human neuronal serotonergic hNT2.19 cells can be used as a biologic minipump for receiving SCI-related neuropathic pain, but likely requires intraspinal grafts for motor recovery.
AB - Transplant of cells which make biologic agents that can modulate the sensory and motor responses after spinal cord injury (SCI) would be useful to treat pain and paralysis. To address this need for clinically useful human cells, a unique neuronal cell line that synthesizes and secretes/releases the neurotransmitter serotonin (5HT) was isolated. Hind paw tactile allodynia and thermal hyperalgesia induced by severe contusive SCI were potently reversed after lumbar subarachnoid transplant of differentiated cells, but had no effect on open fieldmotor scores, stride length, foot rotation, base of support, or gridwalk footfall errors associatedwith the SCI. The sensory effects appeared 1 week after transplant and did not diminish during the 8-week course of the experiment when grafts were placed 2 weeks after SCI. Many grafted cells were still present and synthesizing 5HT at the end of the study. These data suggest that the human neuronal serotonergic hNT2.19 cells can be used as a biologic minipump for receiving SCI-related neuropathic pain, but likely requires intraspinal grafts for motor recovery.
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U2 - 10.1155/2011/891605
DO - 10.1155/2011/891605
M3 - Article
AN - SCOPUS:84861092003
VL - 2011
JO - Neurology Research International
JF - Neurology Research International
SN - 2090-1852
M1 - 891605
ER -