Sub-basal corneal nerve plexus analysis using a new software technology

Hatim Batawi, Nabeel Shalabi, Madhura Joag, Tulay Sengul, Jorge Rodriguez, Parke T. Green, Mauro Campigotto, Carol Karp, Anat Galor

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Purpose: To study sub-basal corneal nerve plexus (SCNP) parameters by in vivo corneal confocal microscopy using a new software technology and examine the effect of demographics and diabetes mellitus (DM) on corneal nerves morphology. Methods: A Confoscan 4 (Nidek Technologies) was used in this crosssectional study to image the SCNP in 84 right eyes at the Miami Veterans Affairs eye clinic. Images were analyzed using a new semiautomated nerve analysis software program (The Corneal Nerve Analysis tool) which evaluated 9 parameters including nerve fibers length (NFL) and nerve fibers length density (NFLD). The main outcome measure was the examination of SCNP morphology by demographics, comorbidities, and HbA1c level. Results: Interoperator and intraoperator reproducibility were good for the 9 parameters studied (Intraclass Correlations [ICCs] 0.73-0.97). Image variability between two images within the same scan was good for all parameters (ICC 0.66-0.80). Older individuals had lower SCNP parameters with NFL and NFLD negatively correlating with age (r=20.471, and 20.461, respectively, P<0.01 for all). Patients with diabetes had lower mean NFLD 10987.6 mm/mm2 (±3,284.6) and NFL 1,289.5 mm/frame (±387.2) compared with patients without diabetes (mean NFLD 15077.1 mμ/mm2 [±4,261.3] and NFL 1750.0 mμ/frame [±540.7]) (P<0.05 for all). HbA1c levels in patients with diabetes were inversely correlated with NFL and NFLD (r= 20.568, and 20.569, respectively, P<0.05 for all). Conclusions: The Corneal Nerve Analysis tool is a reproducible diagnostic software technique for the analysis of the SCNP with confocal microscopy. Older age, DM, and higher level of HbA1c were associated with a significant reduction in SCNP parameters.

Original languageEnglish (US)
Pages (from-to)S199-S205
JournalEye and Contact Lens
Volume44
Issue number5
DOIs
StatePublished - Jan 1 2018

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Nerve Fibers
Software
Technology
Confocal Microscopy
Diabetes Mellitus
Demography
Veterans
Comorbidity
Outcome Assessment (Health Care)

Keywords

  • Age
  • Confocal microscopy
  • Diabetes
  • Nerve morphology

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Sub-basal corneal nerve plexus analysis using a new software technology. / Batawi, Hatim; Shalabi, Nabeel; Joag, Madhura; Sengul, Tulay; Rodriguez, Jorge; Green, Parke T.; Campigotto, Mauro; Karp, Carol; Galor, Anat.

In: Eye and Contact Lens, Vol. 44, No. 5, 01.01.2018, p. S199-S205.

Research output: Contribution to journalArticle

Batawi, H, Shalabi, N, Joag, M, Sengul, T, Rodriguez, J, Green, PT, Campigotto, M, Karp, C & Galor, A 2018, 'Sub-basal corneal nerve plexus analysis using a new software technology', Eye and Contact Lens, vol. 44, no. 5, pp. S199-S205. https://doi.org/10.1097/ICL.0000000000000375
Batawi, Hatim ; Shalabi, Nabeel ; Joag, Madhura ; Sengul, Tulay ; Rodriguez, Jorge ; Green, Parke T. ; Campigotto, Mauro ; Karp, Carol ; Galor, Anat. / Sub-basal corneal nerve plexus analysis using a new software technology. In: Eye and Contact Lens. 2018 ; Vol. 44, No. 5. pp. S199-S205.
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