Abstract
Purpose: To study sub-basal corneal nerve plexus (SCNP) parameters by in vivo corneal confocal microscopy using a new software technology and examine the effect of demographics and diabetes mellitus (DM) on corneal nerves morphology. Methods: A Confoscan 4 (Nidek Technologies) was used in this crosssectional study to image the SCNP in 84 right eyes at the Miami Veterans Affairs eye clinic. Images were analyzed using a new semiautomated nerve analysis software program (The Corneal Nerve Analysis tool) which evaluated 9 parameters including nerve fibers length (NFL) and nerve fibers length density (NFLD). The main outcome measure was the examination of SCNP morphology by demographics, comorbidities, and HbA1c level. Results: Interoperator and intraoperator reproducibility were good for the 9 parameters studied (Intraclass Correlations [ICCs] 0.73-0.97). Image variability between two images within the same scan was good for all parameters (ICC 0.66-0.80). Older individuals had lower SCNP parameters with NFL and NFLD negatively correlating with age (r=20.471, and 20.461, respectively, P<0.01 for all). Patients with diabetes had lower mean NFLD 10987.6 mm/mm2 (±3,284.6) and NFL 1,289.5 mm/frame (±387.2) compared with patients without diabetes (mean NFLD 15077.1 mμ/mm2 [±4,261.3] and NFL 1750.0 mμ/frame [±540.7]) (P<0.05 for all). HbA1c levels in patients with diabetes were inversely correlated with NFL and NFLD (r= 20.568, and 20.569, respectively, P<0.05 for all). Conclusions: The Corneal Nerve Analysis tool is a reproducible diagnostic software technique for the analysis of the SCNP with confocal microscopy. Older age, DM, and higher level of HbA1c were associated with a significant reduction in SCNP parameters.
| Original language | English (US) |
|---|---|
| Pages (from-to) | S199-S205 |
| Journal | Eye and Contact Lens |
| Volume | 44 |
| Issue number | 5 |
| DOIs | |
| State | Published - Jan 1 2018 |
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Keywords
- Age
- Confocal microscopy
- Diabetes
- Nerve morphology
ASJC Scopus subject areas
- Ophthalmology
Cite this
Sub-basal corneal nerve plexus analysis using a new software technology. / Batawi, Hatim; Shalabi, Nabeel; Joag, Madhura; Sengul, Tulay; Rodriguez, Jorge; Green, Parke T.; Campigotto, Mauro; Karp, Carol; Galor, Anat.
In: Eye and Contact Lens, Vol. 44, No. 5, 01.01.2018, p. S199-S205.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sub-basal corneal nerve plexus analysis using a new software technology
AU - Batawi, Hatim
AU - Shalabi, Nabeel
AU - Joag, Madhura
AU - Sengul, Tulay
AU - Rodriguez, Jorge
AU - Green, Parke T.
AU - Campigotto, Mauro
AU - Karp, Carol
AU - Galor, Anat
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Purpose: To study sub-basal corneal nerve plexus (SCNP) parameters by in vivo corneal confocal microscopy using a new software technology and examine the effect of demographics and diabetes mellitus (DM) on corneal nerves morphology. Methods: A Confoscan 4 (Nidek Technologies) was used in this crosssectional study to image the SCNP in 84 right eyes at the Miami Veterans Affairs eye clinic. Images were analyzed using a new semiautomated nerve analysis software program (The Corneal Nerve Analysis tool) which evaluated 9 parameters including nerve fibers length (NFL) and nerve fibers length density (NFLD). The main outcome measure was the examination of SCNP morphology by demographics, comorbidities, and HbA1c level. Results: Interoperator and intraoperator reproducibility were good for the 9 parameters studied (Intraclass Correlations [ICCs] 0.73-0.97). Image variability between two images within the same scan was good for all parameters (ICC 0.66-0.80). Older individuals had lower SCNP parameters with NFL and NFLD negatively correlating with age (r=20.471, and 20.461, respectively, P<0.01 for all). Patients with diabetes had lower mean NFLD 10987.6 mm/mm2 (±3,284.6) and NFL 1,289.5 mm/frame (±387.2) compared with patients without diabetes (mean NFLD 15077.1 mμ/mm2 [±4,261.3] and NFL 1750.0 mμ/frame [±540.7]) (P<0.05 for all). HbA1c levels in patients with diabetes were inversely correlated with NFL and NFLD (r= 20.568, and 20.569, respectively, P<0.05 for all). Conclusions: The Corneal Nerve Analysis tool is a reproducible diagnostic software technique for the analysis of the SCNP with confocal microscopy. Older age, DM, and higher level of HbA1c were associated with a significant reduction in SCNP parameters.
AB - Purpose: To study sub-basal corneal nerve plexus (SCNP) parameters by in vivo corneal confocal microscopy using a new software technology and examine the effect of demographics and diabetes mellitus (DM) on corneal nerves morphology. Methods: A Confoscan 4 (Nidek Technologies) was used in this crosssectional study to image the SCNP in 84 right eyes at the Miami Veterans Affairs eye clinic. Images were analyzed using a new semiautomated nerve analysis software program (The Corneal Nerve Analysis tool) which evaluated 9 parameters including nerve fibers length (NFL) and nerve fibers length density (NFLD). The main outcome measure was the examination of SCNP morphology by demographics, comorbidities, and HbA1c level. Results: Interoperator and intraoperator reproducibility were good for the 9 parameters studied (Intraclass Correlations [ICCs] 0.73-0.97). Image variability between two images within the same scan was good for all parameters (ICC 0.66-0.80). Older individuals had lower SCNP parameters with NFL and NFLD negatively correlating with age (r=20.471, and 20.461, respectively, P<0.01 for all). Patients with diabetes had lower mean NFLD 10987.6 mm/mm2 (±3,284.6) and NFL 1,289.5 mm/frame (±387.2) compared with patients without diabetes (mean NFLD 15077.1 mμ/mm2 [±4,261.3] and NFL 1750.0 mμ/frame [±540.7]) (P<0.05 for all). HbA1c levels in patients with diabetes were inversely correlated with NFL and NFLD (r= 20.568, and 20.569, respectively, P<0.05 for all). Conclusions: The Corneal Nerve Analysis tool is a reproducible diagnostic software technique for the analysis of the SCNP with confocal microscopy. Older age, DM, and higher level of HbA1c were associated with a significant reduction in SCNP parameters.
KW - Age
KW - Confocal microscopy
KW - Diabetes
KW - Nerve morphology
UR - http://www.scopus.com/inward/record.url?scp=85016092339&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85016092339&partnerID=8YFLogxK
U2 - 10.1097/ICL.0000000000000375
DO - 10.1097/ICL.0000000000000375
M3 - Article
VL - 44
SP - S199-S205
JO - Eye and Contact Lense
JF - Eye and Contact Lense
SN - 1542-2321
IS - 5
ER -