Study of the development and evolution of neointestine in a rat model

W. De Faria, P. Tryphonopoulos, G. Kleiner, Sergio F Santiago, C. Gandia, Phillip Ruiz, A. Tzakis

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The implantation of fragmented rat intestinal epithelium into the omentum of syngeneic animals results in the formation of a cyst containing neointestine. The purpose of our project was to study the evolution of this neointestine-containing cyst over time. Harvested jejunum and ileum of neonatal DA rats (6 to 8 days old) was digested with collagenase type XI and dispase at room temperature. The resulting organoid units, containing clusters of intestinal epithelium with stem cells were seeded onto a polyglactin polymer mesh (100,000 units per mesh). The absorbable mesh was implanted in the omentum or peritoneal wall of an adult syngeneic animal. Animals were sacrificed at weekly intervals to harvest the neointestinal cysts. The lumen of the neointestine cysts was full of mucous while the wall of the cyst was covered by intestinal mucosa. H&E staining of the cyst demonstrated the morphology of intestinal epithelium; PAS staining identified goblet cells. The size of the cyst was maximal between 4 and 8 weeks postimplantation tending to regress thereafter. Neointestinal cysts are a consistent finding after implantation of intestinal epithelium organoids into the omentum or peritoneal wall in the rat model. The cysts reach a maximal size at 4 to 8 weeks postimplantation, tending to regress thereafter.

Original languageEnglish
Pages (from-to)375-376
Number of pages2
JournalTransplantation Proceedings
Volume36
Issue number2
DOIs
StatePublished - Mar 1 2004

Fingerprint

Cysts
Intestinal Mucosa
Omentum
Organoids
Staining and Labeling
Polyglactin 910
Goblet Cells
Collagenases
Jejunum
Ileum
Polymers
Stem Cells
Temperature

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Cite this

Study of the development and evolution of neointestine in a rat model. / De Faria, W.; Tryphonopoulos, P.; Kleiner, G.; Santiago, Sergio F; Gandia, C.; Ruiz, Phillip; Tzakis, A.

In: Transplantation Proceedings, Vol. 36, No. 2, 01.03.2004, p. 375-376.

Research output: Contribution to journalArticle

De Faria, W. ; Tryphonopoulos, P. ; Kleiner, G. ; Santiago, Sergio F ; Gandia, C. ; Ruiz, Phillip ; Tzakis, A. / Study of the development and evolution of neointestine in a rat model. In: Transplantation Proceedings. 2004 ; Vol. 36, No. 2. pp. 375-376.
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