TY - JOUR
T1 - Study of the Alpha-l-Fucosidase Langmuir Monolayer at the Air-Water Interface
AU - Waidely, Eric
AU - Al-Youbi, Abdulrahman O.
AU - Bashammakh, Abdulaziz S.
AU - El-Shahawi, Mohammad S.
AU - Leblanc, Roger M.
PY - 2016/12/22
Y1 - 2016/12/22
N2 - Alpha-l-fucosidase is a known biomarker for hepatocellular carcinoma that has shown great potential in diagnostics. Most of the focus for this enzyme has been on the free form found in serum; however, little is known of the properties of the minor portion of membrane-bound alpha-l-fucosidase. To better understand the properties of membrane-bound alpha-l-fucosidase, this enzyme was surveyed at the air-water interface. Alpha-l-fucosidase is able to form a stable Langmuir monolayer, which was confirmed through surface-pressure and surface-potential area isotherms, as well as infrared reflection-absorption spectroscopy (IRRAS). Furthermore, an interaction between the alpha-l-fucosidase Langmuir monolayer and a specific antibody for this enzyme, FUCA2, was observed.
AB - Alpha-l-fucosidase is a known biomarker for hepatocellular carcinoma that has shown great potential in diagnostics. Most of the focus for this enzyme has been on the free form found in serum; however, little is known of the properties of the minor portion of membrane-bound alpha-l-fucosidase. To better understand the properties of membrane-bound alpha-l-fucosidase, this enzyme was surveyed at the air-water interface. Alpha-l-fucosidase is able to form a stable Langmuir monolayer, which was confirmed through surface-pressure and surface-potential area isotherms, as well as infrared reflection-absorption spectroscopy (IRRAS). Furthermore, an interaction between the alpha-l-fucosidase Langmuir monolayer and a specific antibody for this enzyme, FUCA2, was observed.
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U2 - 10.1021/acs.jpcb.6b09094
DO - 10.1021/acs.jpcb.6b09094
M3 - Article
C2 - 27936789
AN - SCOPUS:85045862098
VL - 120
SP - 12843
EP - 12849
JO - Journal of Physical Chemistry B Materials
JF - Journal of Physical Chemistry B Materials
SN - 1520-6106
IS - 50
ER -