Study of the Alpha-l-Fucosidase Langmuir Monolayer at the Air-Water Interface

Eric Waidely; Abdulrahman O. Al-Youbi; Abdulaziz S. Bashammakh; Mohammad S. El-Shahawi; Roger M. Leblanc

doi:10.1021/acs.jpcb.6b09094

Study of the Alpha-l-Fucosidase Langmuir Monolayer at the Air-Water Interface

Eric Waidely, Abdulrahman O. Al-Youbi, Abdulaziz S. Bashammakh, Mohammad S. El-Shahawi, Roger M. Leblanc

Chemistry

Research output: Contribution to journal › Article

1 Scopus citations

Abstract

Alpha-l-fucosidase is a known biomarker for hepatocellular carcinoma that has shown great potential in diagnostics. Most of the focus for this enzyme has been on the free form found in serum; however, little is known of the properties of the minor portion of membrane-bound alpha-l-fucosidase. To better understand the properties of membrane-bound alpha-l-fucosidase, this enzyme was surveyed at the air-water interface. Alpha-l-fucosidase is able to form a stable Langmuir monolayer, which was confirmed through surface-pressure and surface-potential area isotherms, as well as infrared reflection-absorption spectroscopy (IRRAS). Furthermore, an interaction between the alpha-l-fucosidase Langmuir monolayer and a specific antibody for this enzyme, FUCA2, was observed.

Original language	English (US)
Pages (from-to)	12843-12849
Number of pages	7
Journal	The journal of physical chemistry. B
Volume	120
Issue number	50
DOIs	https://doi.org/10.1021/acs.jpcb.6b09094
State	Published - Dec 22 2016

ASJC Scopus subject areas

Physical and Theoretical Chemistry
Surfaces, Coatings and Films
Materials Chemistry

Access to Document

10.1021/acs.jpcb.6b09094

Fingerprint Dive into the research topics of 'Study of the Alpha-l-Fucosidase Langmuir Monolayer at the Air-Water Interface'. Together they form a unique fingerprint.

Cite this

Waidely, E., Al-Youbi, A. O., Bashammakh, A. S., El-Shahawi, M. S., & Leblanc, R. M. (2016). Study of the Alpha-l-Fucosidase Langmuir Monolayer at the Air-Water Interface. The journal of physical chemistry. B, 120(50), 12843-12849. https://doi.org/10.1021/acs.jpcb.6b09094

@article{49823974309f4a87b5c8a5c9ddc34ce0,

title = "Study of the Alpha-l-Fucosidase Langmuir Monolayer at the Air-Water Interface",

abstract = "Alpha-l-fucosidase is a known biomarker for hepatocellular carcinoma that has shown great potential in diagnostics. Most of the focus for this enzyme has been on the free form found in serum; however, little is known of the properties of the minor portion of membrane-bound alpha-l-fucosidase. To better understand the properties of membrane-bound alpha-l-fucosidase, this enzyme was surveyed at the air-water interface. Alpha-l-fucosidase is able to form a stable Langmuir monolayer, which was confirmed through surface-pressure and surface-potential area isotherms, as well as infrared reflection-absorption spectroscopy (IRRAS). Furthermore, an interaction between the alpha-l-fucosidase Langmuir monolayer and a specific antibody for this enzyme, FUCA2, was observed.",

author = "Eric Waidely and Al-Youbi, {Abdulrahman O.} and Bashammakh, {Abdulaziz S.} and El-Shahawi, {Mohammad S.} and Leblanc, {Roger M.}",

year = "2016",

month = dec,

day = "22",

doi = "10.1021/acs.jpcb.6b09094",

language = "English (US)",

volume = "120",

pages = "12843--12849",

journal = "Journal of Physical Chemistry B Materials",

issn = "1520-6106",

publisher = "American Chemical Society",

number = "50",

}

TY - JOUR

T1 - Study of the Alpha-l-Fucosidase Langmuir Monolayer at the Air-Water Interface

AU - Waidely, Eric

AU - Al-Youbi, Abdulrahman O.

AU - Bashammakh, Abdulaziz S.

AU - El-Shahawi, Mohammad S.

AU - Leblanc, Roger M.

PY - 2016/12/22

Y1 - 2016/12/22

N2 - Alpha-l-fucosidase is a known biomarker for hepatocellular carcinoma that has shown great potential in diagnostics. Most of the focus for this enzyme has been on the free form found in serum; however, little is known of the properties of the minor portion of membrane-bound alpha-l-fucosidase. To better understand the properties of membrane-bound alpha-l-fucosidase, this enzyme was surveyed at the air-water interface. Alpha-l-fucosidase is able to form a stable Langmuir monolayer, which was confirmed through surface-pressure and surface-potential area isotherms, as well as infrared reflection-absorption spectroscopy (IRRAS). Furthermore, an interaction between the alpha-l-fucosidase Langmuir monolayer and a specific antibody for this enzyme, FUCA2, was observed.

AB - Alpha-l-fucosidase is a known biomarker for hepatocellular carcinoma that has shown great potential in diagnostics. Most of the focus for this enzyme has been on the free form found in serum; however, little is known of the properties of the minor portion of membrane-bound alpha-l-fucosidase. To better understand the properties of membrane-bound alpha-l-fucosidase, this enzyme was surveyed at the air-water interface. Alpha-l-fucosidase is able to form a stable Langmuir monolayer, which was confirmed through surface-pressure and surface-potential area isotherms, as well as infrared reflection-absorption spectroscopy (IRRAS). Furthermore, an interaction between the alpha-l-fucosidase Langmuir monolayer and a specific antibody for this enzyme, FUCA2, was observed.

UR - http://www.scopus.com/inward/record.url?scp=85045862098&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85045862098&partnerID=8YFLogxK

U2 - 10.1021/acs.jpcb.6b09094

DO - 10.1021/acs.jpcb.6b09094

M3 - Article

C2 - 27936789

AN - SCOPUS:85045862098

VL - 120

SP - 12843

EP - 12849

JO - Journal of Physical Chemistry B Materials

JF - Journal of Physical Chemistry B Materials

SN - 1520-6106

IS - 50

ER -