Study of the Alpha-l-Fucosidase Langmuir Monolayer at the Air-Water Interface

Eric Waidely; Abdulrahman O. Al-Youbi; Abdulaziz S. Bashammakh; Mohammad S. El-Shahawi; Roger M. Leblanc

doi:10.1021/acs.jpcb.6b09094

Study of the Alpha-l-Fucosidase Langmuir Monolayer at the Air-Water Interface

Eric Waidely, Abdulrahman O. Al-Youbi, Abdulaziz S. Bashammakh, Mohammad S. El-Shahawi, Roger M. Leblanc

Chemistry

Research output: Contribution to journal › Article

1 Scopus citations

Abstract

Alpha-l-fucosidase is a known biomarker for hepatocellular carcinoma that has shown great potential in diagnostics. Most of the focus for this enzyme has been on the free form found in serum; however, little is known of the properties of the minor portion of membrane-bound alpha-l-fucosidase. To better understand the properties of membrane-bound alpha-l-fucosidase, this enzyme was surveyed at the air-water interface. Alpha-l-fucosidase is able to form a stable Langmuir monolayer, which was confirmed through surface-pressure and surface-potential area isotherms, as well as infrared reflection-absorption spectroscopy (IRRAS). Furthermore, an interaction between the alpha-l-fucosidase Langmuir monolayer and a specific antibody for this enzyme, FUCA2, was observed.

Original language	English (US)
Pages (from-to)	12843-12849
Number of pages	7
Journal	The journal of physical chemistry. B
Volume	120
Issue number	50
DOIs	https://doi.org/10.1021/acs.jpcb.6b09094
State	Published - Dec 22 2016

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ASJC Scopus subject areas

Physical and Theoretical Chemistry
Surfaces, Coatings and Films
Materials Chemistry

Cite this

Waidely, E., Al-Youbi, A. O., Bashammakh, A. S., El-Shahawi, M. S., & Leblanc, R. M. (2016). Study of the Alpha-l-Fucosidase Langmuir Monolayer at the Air-Water Interface. The journal of physical chemistry. B, 120(50), 12843-12849. https://doi.org/10.1021/acs.jpcb.6b09094

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AB - Alpha-l-fucosidase is a known biomarker for hepatocellular carcinoma that has shown great potential in diagnostics. Most of the focus for this enzyme has been on the free form found in serum; however, little is known of the properties of the minor portion of membrane-bound alpha-l-fucosidase. To better understand the properties of membrane-bound alpha-l-fucosidase, this enzyme was surveyed at the air-water interface. Alpha-l-fucosidase is able to form a stable Langmuir monolayer, which was confirmed through surface-pressure and surface-potential area isotherms, as well as infrared reflection-absorption spectroscopy (IRRAS). Furthermore, an interaction between the alpha-l-fucosidase Langmuir monolayer and a specific antibody for this enzyme, FUCA2, was observed.

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Access to Document

10.1021/acs.jpcb.6b09094