Phycobilisomes oriented in stretched polymer films exhibit anistropy of absorption and fluorescence. Up to now it was not clear in which way, by the deformation of their shape or by orientation of undeformed phycobilisome disk, this anisotropy of chromophore absorption and emission is reached. The photographs of two different kinds of phycobilisomes obtained from cyanobacteria Tolypothrix tenuis and Mastigocladus laminosus were taken using a fluorescence microscope. From these photographs follows that phycobilisomes form in polymer big clusters and that these clusters are deformed as a result of stretching. It shows that predominantly the interaction between phycobilisomes is responsible for their orientation. Probably in clusters most phycobilisomes are oriented with disk diameter along the direction of film stretching. The fluoresence decay curves were recorded for Mastigocladus laminosus phycobilisomes embedded in PVA films. At least biexponential decay law has to be assumed. Calculated lifetimes are discussed in terms of energy transfer from primarily excited to fluorescent chromophores.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and biophysical research communications|
|State||Published - Nov 30 1993|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology