Studies on the properties of hypothalamic luteinizing hormone-releasing hormone.

Y. Baba, A. Arimura, Andrew V Schally

Research output: Contribution to journalArticle

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Abstract

The effects of various chemical and enzymatic treatments on the biological activity of porcine luteinizing hormone-releasing hormone (LH-RH) are described. This experiment was performed before the elucidation of the structure of LH-RH. LH-RH activity was abolished by the following endopeptidases: chymotrypsin, subtilisin, papain, and thermolysin, but not by pepsin or trypsin. Exopeptidases did not affect LH-RH activity, but a purified preparation of pyrolidone carbosylpeptidase did. The amino acid sequence of LH-RH/FSH-RH was established to be (pyro)Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-Amine. This decapeptide lacks both the Amine terminus and the COOH terminus. Its Amine-terminal dipeptide sequence,(pyro)Glu-His, is similar to that of tyrotropin-releasing hormone. The lack of inactivation by the exopeptidases is in good agreement with these findings. Treatment with various chemical reagents showed that tyrosine, histidine, tryptophan, and arginine in LH-RH are important for its biological activity. Nitrous acid and Edman degradation did not inactivate LH-RH. These results are also in agreement with the determined structure of LH-RH. This hormone showed a high follicle-stimulating hormone-releasing hormone (FSH-RH) activity. The inactivation of LH-RH was always accompanied by a loss of FSH-RH activity. These experiments also shed some light on the structure-activity relationship of this hormone.

Original languageEnglish
Pages (from-to)7581-7585
Number of pages5
JournalJournal of Biological Chemistry
Volume246
Issue number24
StatePublished - Dec 25 1971
Externally publishedYes

Fingerprint

Hypothalamic Hormones
Gonadotropin-Releasing Hormone
Hormones
Follicle Stimulating Hormone
Exopeptidases
Amines
Bioactivity
Nitrous Acid
Thermolysin
Subtilisin
Endopeptidases
Papain
Dipeptides
Pepsin A
Chymotrypsin
Structure-Activity Relationship
Histidine
Tryptophan
Trypsin
Tyrosine

ASJC Scopus subject areas

  • Biochemistry

Cite this

Studies on the properties of hypothalamic luteinizing hormone-releasing hormone. / Baba, Y.; Arimura, A.; Schally, Andrew V.

In: Journal of Biological Chemistry, Vol. 246, No. 24, 25.12.1971, p. 7581-7585.

Research output: Contribution to journalArticle

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