Studies on the mechanism of action of a targeted chemotherapeutic drug in living cancer cells by two photon laser scanning microspectrofluorometry

Xiaopeng Wang, Haridas E. Pudavar, Rakesh Kapoor, Linda J. Krebs, Earl J. Bergey, Charles Liebow, Paras N. Prasad, Attila Nagy, Andrew V. Schally

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

In this study, we present a spectroscopic study of the entry pattern of a chemotherapeutic drug (AN-152) and its carrier hormone ([D-Lys6]LH-RH) into living cancer cells, with the help of our two-photon probes and a home-built localized microspectrofluorometer coupled with two photon laser scanning microscope (TPLSM). Due to the inherent localization ability of TPLSM, we were able to identify the drug and carrier location in different compartments of the cancer cells in vitro. The apparent doxorubicin-assisted nucleic accumulation of AN-152 suggests a possible nuclear action of the drug on cell proliferation.

Original languageEnglish (US)
Pages (from-to)319-325
Number of pages7
JournalJournal of Biomedical Optics
Volume6
Issue number3
DOIs
StatePublished - Jul 2001
Externally publishedYes

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Keywords

  • Doxorubicin
  • Drug trafficking
  • LH-RH
  • Multicolor imaging
  • TPLSMF
  • Two-photon laser scanning microfluorometry

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Radiology Nuclear Medicine and imaging
  • Atomic and Molecular Physics, and Optics
  • Radiological and Ultrasound Technology

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