Studies on the biology of fish bone-II bone matrix changes during resorption

Roy E Weiss, Norimitsu Watabe

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

1. 1. The present investigation describes changes in the water-soluble organic matrix during bone resorption in the acellular-boned fish Tilapia macrocephala. Bone resorption was induced after scale removal. 2. 2. Bone carbohydrate concentration decreased 49.2% in 2 days and 93.7% 6 days after scale removal. 3. 3. Bone calcium concentration remained the same at 2 and 4 days but decreased by 63% 6 days after scale removal. 4. 4. The soluble organic matrix was found to be altered prior to mineral removal. 5. 5. In normal bone matrix, two heat-labile, calcium-binding components were identified: One (molecular weight 15,000), not sensitive to beta-glucuronidase, and the other (molecular weight 6000), which was sensitive to beta-glucuronidase. Uronic acid was found to be associated with these components. 6. 6. In resorbing bone matrix only one calcium binding component (molecular weight 15,000) was identified.

Original languageEnglish (US)
Pages (from-to)245-251
Number of pages7
JournalComparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
Volume61
Issue number2
DOIs
StatePublished - 1978
Externally publishedYes

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Bone Matrix
Fish
Bone
Fishes
Molecular Weight
Glucuronidase
Descaling
Bone Resorption
Calcium
Bone and Bones
Uronic Acids
Tilapia
Molecular weight
Minerals
Hot Temperature
Carbohydrates
Water

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Biochemistry

Cite this

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abstract = "1. 1. The present investigation describes changes in the water-soluble organic matrix during bone resorption in the acellular-boned fish Tilapia macrocephala. Bone resorption was induced after scale removal. 2. 2. Bone carbohydrate concentration decreased 49.2{\%} in 2 days and 93.7{\%} 6 days after scale removal. 3. 3. Bone calcium concentration remained the same at 2 and 4 days but decreased by 63{\%} 6 days after scale removal. 4. 4. The soluble organic matrix was found to be altered prior to mineral removal. 5. 5. In normal bone matrix, two heat-labile, calcium-binding components were identified: One (molecular weight 15,000), not sensitive to beta-glucuronidase, and the other (molecular weight 6000), which was sensitive to beta-glucuronidase. Uronic acid was found to be associated with these components. 6. 6. In resorbing bone matrix only one calcium binding component (molecular weight 15,000) was identified.",
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N2 - 1. 1. The present investigation describes changes in the water-soluble organic matrix during bone resorption in the acellular-boned fish Tilapia macrocephala. Bone resorption was induced after scale removal. 2. 2. Bone carbohydrate concentration decreased 49.2% in 2 days and 93.7% 6 days after scale removal. 3. 3. Bone calcium concentration remained the same at 2 and 4 days but decreased by 63% 6 days after scale removal. 4. 4. The soluble organic matrix was found to be altered prior to mineral removal. 5. 5. In normal bone matrix, two heat-labile, calcium-binding components were identified: One (molecular weight 15,000), not sensitive to beta-glucuronidase, and the other (molecular weight 6000), which was sensitive to beta-glucuronidase. Uronic acid was found to be associated with these components. 6. 6. In resorbing bone matrix only one calcium binding component (molecular weight 15,000) was identified.

AB - 1. 1. The present investigation describes changes in the water-soluble organic matrix during bone resorption in the acellular-boned fish Tilapia macrocephala. Bone resorption was induced after scale removal. 2. 2. Bone carbohydrate concentration decreased 49.2% in 2 days and 93.7% 6 days after scale removal. 3. 3. Bone calcium concentration remained the same at 2 and 4 days but decreased by 63% 6 days after scale removal. 4. 4. The soluble organic matrix was found to be altered prior to mineral removal. 5. 5. In normal bone matrix, two heat-labile, calcium-binding components were identified: One (molecular weight 15,000), not sensitive to beta-glucuronidase, and the other (molecular weight 6000), which was sensitive to beta-glucuronidase. Uronic acid was found to be associated with these components. 6. 6. In resorbing bone matrix only one calcium binding component (molecular weight 15,000) was identified.

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