Studies on TGF-β1 gene expression in the intima of the human aorta in regions with high and low probability of developing atherosclerotic lesions

P. Borkowski, M. J. Robinson, J. W. Kusiak, A. Borkowski, C. Brathwaite, W. J. Mergner

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Certain regions of the human aorta are at greater risk for early and more severe atherosclerotic lesions development than others. Cornhill and coworkers (Cornhill FJ et al.: Arteriosclerosis 5:415, 1985) created maps for the probability of-developing atherosclerosis defining the high-probability region (HPR) in the dorsal descending thoracic aorta and the low-probability region (LPR) in the ventral descending thoracic aorta. Our study examines the hypothesis that transforming growth factor β-1 (TGF-β1), a well-known suppressor of growth and function in many human cell lines, is one of the inhibitors of human atherogenesis. The present experiment analyzes the expression of mRNA for TGF-β1 in both the HPR and the LPR of aortas from young (age 17 to 25 y) males of black (n = 8) and white (n = 7) race. The level of TGF-β1 gene expression was assessed in the aortic intima in both the HPR and the LPR, using National Institutes of Health Image 1.47, an Apple Macintosh application capable of digital image processing, analysis, and morphometric measurement. There was significantly lower (P = 0.002, α = 0.05) TGF-β1 gene expression in the HPR than in the LPR in the 22- to 25-y age group. There was no significant difference in the 17- to 21-y age group and between the HPR and the LPR in the entire study group. We conclude that the decreased intimal TGF-1 gene expression suggests areas of lowered inhibitory activity for growth of human atherosclerotic plaques and therefore may have a direct relationship with the increased development of raised lesions in the HPR of the human aorta.

Original languageEnglish (US)
Pages (from-to)478-482
Number of pages5
JournalModern Pathology
Volume8
Issue number5
StatePublished - Jan 1 1995

Keywords

  • Aorta
  • Atherosclerosis
  • Computerized morphometry
  • Human
  • In situ hybridizatioa
  • mRNA
  • Transforming growth factor β-1

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Borkowski, P., Robinson, M. J., Kusiak, J. W., Borkowski, A., Brathwaite, C., & Mergner, W. J. (1995). Studies on TGF-β1 gene expression in the intima of the human aorta in regions with high and low probability of developing atherosclerotic lesions. Modern Pathology, 8(5), 478-482.