Studies on in-vivo priming of the trinitrophenyl-reactive cytotoxic effector-cell system. III. Are helper T cells involved in the in-vitro generation of cross-reactive as well as restricted cytotoxic responses?

H. Fujiwara, Robert B Levy, G. M. Shearer

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The present study investigates the role of helper cells from trinitrophenyl(TNP)-primed mice in the generation of cytotoxic T lymphocytes (CTL) which lyse TNP-modified syngeneic (TNP-self) targets and cross-reactively lyse TNP-allogeneic target cells. Anti-TNP CTL activities generated from primed (either by intraperitoneal injection of TNP-syngeneic cells or by skin painting with trinitrochlorobenzene) and unprimed spleen cells were compared. When effector populations with comparable lytic activities on TNP-self targets were tested against allogeneic TNP-modified targets, only the in vivo primed effector cells displayed significant cytotoxicity. Since radioresistant helper cells have been found to enhance the anti-TNP-self CTL response, the question was raised whether this helper population could be shown to be involved in the enhanced TNP-allogeneic cross-reactive lysis. Normal spleen cells co-cultured with radioresistant helper cells failed to induce any detectable lysis on TNP-allogeneic targets under conditions for which these helper cells did enhance the lysis detected on TNP-self targets. These results that suggest triggering of such TNP-cross-reactive effector cells during the in vivo priming stage is responsible for the in vitro generation of cross-reactivity.

Original languageEnglish
Pages (from-to)472-478
Number of pages7
JournalImmunobiology
Volume160
Issue number5
StatePublished - Dec 1 1981
Externally publishedYes

Fingerprint

Helper-Inducer T-Lymphocytes
Cytotoxic T-Lymphocytes
Spleen
Picryl Chloride
Paintings
Intraperitoneal Injections
Population
Cultured Cells
In Vitro Techniques
Skin

ASJC Scopus subject areas

  • Immunology

Cite this

@article{74035e18c9e64482a17ba2e80be3c81e,
title = "Studies on in-vivo priming of the trinitrophenyl-reactive cytotoxic effector-cell system. III. Are helper T cells involved in the in-vitro generation of cross-reactive as well as restricted cytotoxic responses?",
abstract = "The present study investigates the role of helper cells from trinitrophenyl(TNP)-primed mice in the generation of cytotoxic T lymphocytes (CTL) which lyse TNP-modified syngeneic (TNP-self) targets and cross-reactively lyse TNP-allogeneic target cells. Anti-TNP CTL activities generated from primed (either by intraperitoneal injection of TNP-syngeneic cells or by skin painting with trinitrochlorobenzene) and unprimed spleen cells were compared. When effector populations with comparable lytic activities on TNP-self targets were tested against allogeneic TNP-modified targets, only the in vivo primed effector cells displayed significant cytotoxicity. Since radioresistant helper cells have been found to enhance the anti-TNP-self CTL response, the question was raised whether this helper population could be shown to be involved in the enhanced TNP-allogeneic cross-reactive lysis. Normal spleen cells co-cultured with radioresistant helper cells failed to induce any detectable lysis on TNP-allogeneic targets under conditions for which these helper cells did enhance the lysis detected on TNP-self targets. These results that suggest triggering of such TNP-cross-reactive effector cells during the in vivo priming stage is responsible for the in vitro generation of cross-reactivity.",
author = "H. Fujiwara and Levy, {Robert B} and Shearer, {G. M.}",
year = "1981",
month = "12",
day = "1",
language = "English",
volume = "160",
pages = "472--478",
journal = "Immunobiology",
issn = "0171-2985",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "5",

}

TY - JOUR

T1 - Studies on in-vivo priming of the trinitrophenyl-reactive cytotoxic effector-cell system. III. Are helper T cells involved in the in-vitro generation of cross-reactive as well as restricted cytotoxic responses?

AU - Fujiwara, H.

AU - Levy, Robert B

AU - Shearer, G. M.

PY - 1981/12/1

Y1 - 1981/12/1

N2 - The present study investigates the role of helper cells from trinitrophenyl(TNP)-primed mice in the generation of cytotoxic T lymphocytes (CTL) which lyse TNP-modified syngeneic (TNP-self) targets and cross-reactively lyse TNP-allogeneic target cells. Anti-TNP CTL activities generated from primed (either by intraperitoneal injection of TNP-syngeneic cells or by skin painting with trinitrochlorobenzene) and unprimed spleen cells were compared. When effector populations with comparable lytic activities on TNP-self targets were tested against allogeneic TNP-modified targets, only the in vivo primed effector cells displayed significant cytotoxicity. Since radioresistant helper cells have been found to enhance the anti-TNP-self CTL response, the question was raised whether this helper population could be shown to be involved in the enhanced TNP-allogeneic cross-reactive lysis. Normal spleen cells co-cultured with radioresistant helper cells failed to induce any detectable lysis on TNP-allogeneic targets under conditions for which these helper cells did enhance the lysis detected on TNP-self targets. These results that suggest triggering of such TNP-cross-reactive effector cells during the in vivo priming stage is responsible for the in vitro generation of cross-reactivity.

AB - The present study investigates the role of helper cells from trinitrophenyl(TNP)-primed mice in the generation of cytotoxic T lymphocytes (CTL) which lyse TNP-modified syngeneic (TNP-self) targets and cross-reactively lyse TNP-allogeneic target cells. Anti-TNP CTL activities generated from primed (either by intraperitoneal injection of TNP-syngeneic cells or by skin painting with trinitrochlorobenzene) and unprimed spleen cells were compared. When effector populations with comparable lytic activities on TNP-self targets were tested against allogeneic TNP-modified targets, only the in vivo primed effector cells displayed significant cytotoxicity. Since radioresistant helper cells have been found to enhance the anti-TNP-self CTL response, the question was raised whether this helper population could be shown to be involved in the enhanced TNP-allogeneic cross-reactive lysis. Normal spleen cells co-cultured with radioresistant helper cells failed to induce any detectable lysis on TNP-allogeneic targets under conditions for which these helper cells did enhance the lysis detected on TNP-self targets. These results that suggest triggering of such TNP-cross-reactive effector cells during the in vivo priming stage is responsible for the in vitro generation of cross-reactivity.

UR - http://www.scopus.com/inward/record.url?scp=0019835805&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019835805&partnerID=8YFLogxK

M3 - Article

C2 - 6978282

AN - SCOPUS:0019835805

VL - 160

SP - 472

EP - 478

JO - Immunobiology

JF - Immunobiology

SN - 0171-2985

IS - 5

ER -