Studies of the developing chick retina using monoclonal antibody 8A2 that recognizes a novel set of gangliosides

Judy Drazba, Michael Pierce, Vance Lemmon

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A monoclonal antibody, Mab 8A2, that recognizes a novel set of gangliosides was produced by immunizing a mouse with Embryonic Day 14 chick optic nerve. Immunohistochemical studies of the developing chick retina revealed a complex pattern of Mab 8A2 immunoreactivity. Initially, staining is concentrated in the optic fiber layer in the central retina. Later in development, the most intense staining is seen at the periphery of the retina and 8A2 immunoreactivity appears in other retina layers. In the adult retina, 8A2 immunoreactivity is lost from the optic fiber layer but persists in the inner plexiform layer, inner nuclear layer, and outer plexiform layer. Cell culture experiments showed intense staining of neurites from retinal ganglion cells but no staining of Muller cells. Biochemical characterization of the epitope recognized by Mab 8A2 suggests that it includes a 9-O-acetyl group that is present on five different gangliosides. The 8A2 immunoreactive gangliosides are distinct from and have slower mobilities on thin-layer chromatographs than those recognized by Mab D1.1 which recognizes 9-O-acetyl GD3.

Original languageEnglish
Pages (from-to)154-163
Number of pages10
JournalDevelopmental Biology
Volume145
Issue number1
StatePublished - Dec 1 1991
Externally publishedYes

Fingerprint

Gangliosides
Retina
Monoclonal Antibodies
Staining and Labeling
Ependymoglial Cells
Retinal Ganglion Cells
Neurites
Optic Nerve
Epitopes
Cell Culture Techniques

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Studies of the developing chick retina using monoclonal antibody 8A2 that recognizes a novel set of gangliosides. / Drazba, Judy; Pierce, Michael; Lemmon, Vance.

In: Developmental Biology, Vol. 145, No. 1, 01.12.1991, p. 154-163.

Research output: Contribution to journalArticle

@article{4f38296aaa3c4775aa2e1ea96d6718f8,
title = "Studies of the developing chick retina using monoclonal antibody 8A2 that recognizes a novel set of gangliosides",
abstract = "A monoclonal antibody, Mab 8A2, that recognizes a novel set of gangliosides was produced by immunizing a mouse with Embryonic Day 14 chick optic nerve. Immunohistochemical studies of the developing chick retina revealed a complex pattern of Mab 8A2 immunoreactivity. Initially, staining is concentrated in the optic fiber layer in the central retina. Later in development, the most intense staining is seen at the periphery of the retina and 8A2 immunoreactivity appears in other retina layers. In the adult retina, 8A2 immunoreactivity is lost from the optic fiber layer but persists in the inner plexiform layer, inner nuclear layer, and outer plexiform layer. Cell culture experiments showed intense staining of neurites from retinal ganglion cells but no staining of Muller cells. Biochemical characterization of the epitope recognized by Mab 8A2 suggests that it includes a 9-O-acetyl group that is present on five different gangliosides. The 8A2 immunoreactive gangliosides are distinct from and have slower mobilities on thin-layer chromatographs than those recognized by Mab D1.1 which recognizes 9-O-acetyl GD3.",
author = "Judy Drazba and Michael Pierce and Vance Lemmon",
year = "1991",
month = "12",
day = "1",
language = "English",
volume = "145",
pages = "154--163",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Studies of the developing chick retina using monoclonal antibody 8A2 that recognizes a novel set of gangliosides

AU - Drazba, Judy

AU - Pierce, Michael

AU - Lemmon, Vance

PY - 1991/12/1

Y1 - 1991/12/1

N2 - A monoclonal antibody, Mab 8A2, that recognizes a novel set of gangliosides was produced by immunizing a mouse with Embryonic Day 14 chick optic nerve. Immunohistochemical studies of the developing chick retina revealed a complex pattern of Mab 8A2 immunoreactivity. Initially, staining is concentrated in the optic fiber layer in the central retina. Later in development, the most intense staining is seen at the periphery of the retina and 8A2 immunoreactivity appears in other retina layers. In the adult retina, 8A2 immunoreactivity is lost from the optic fiber layer but persists in the inner plexiform layer, inner nuclear layer, and outer plexiform layer. Cell culture experiments showed intense staining of neurites from retinal ganglion cells but no staining of Muller cells. Biochemical characterization of the epitope recognized by Mab 8A2 suggests that it includes a 9-O-acetyl group that is present on five different gangliosides. The 8A2 immunoreactive gangliosides are distinct from and have slower mobilities on thin-layer chromatographs than those recognized by Mab D1.1 which recognizes 9-O-acetyl GD3.

AB - A monoclonal antibody, Mab 8A2, that recognizes a novel set of gangliosides was produced by immunizing a mouse with Embryonic Day 14 chick optic nerve. Immunohistochemical studies of the developing chick retina revealed a complex pattern of Mab 8A2 immunoreactivity. Initially, staining is concentrated in the optic fiber layer in the central retina. Later in development, the most intense staining is seen at the periphery of the retina and 8A2 immunoreactivity appears in other retina layers. In the adult retina, 8A2 immunoreactivity is lost from the optic fiber layer but persists in the inner plexiform layer, inner nuclear layer, and outer plexiform layer. Cell culture experiments showed intense staining of neurites from retinal ganglion cells but no staining of Muller cells. Biochemical characterization of the epitope recognized by Mab 8A2 suggests that it includes a 9-O-acetyl group that is present on five different gangliosides. The 8A2 immunoreactive gangliosides are distinct from and have slower mobilities on thin-layer chromatographs than those recognized by Mab D1.1 which recognizes 9-O-acetyl GD3.

UR - http://www.scopus.com/inward/record.url?scp=0025763640&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025763640&partnerID=8YFLogxK

M3 - Article

VL - 145

SP - 154

EP - 163

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -