Abstract
Tropomyosin was quantitated in embryonic and postembryonic chick leg muscle by a procedure involving acrylamide gel electrophoresis, with subsequent elution of tropomyosin from the gels, followed by adsorption on membrane filters and subsequent extraction and spectrophotometric determination of a dye-tropomyosin complex. Tropomyosin was present at low levels of 20 mg/100 g muscle at 11 days of incubation and increased to 360 mg/100 g muscle at 2 months of development. The accumulation data for tropomyosin parallel those of myosin throughout development. Chicken tropomyosin in the presence of unfractionated chicken troponin (troponin A and B) confers EGTA sensitivity to Mg2+-activated desensitized actomyosin. The presence of troponin B activity in the unfractionated preparation is evident since the troponin preparation in the absence of tropomyosin inhibits superprecipitation of Mg2+-activated desensitized actomyosin. In this respect chick tropomyosin and troponin are similar to the corresponding rabbit protein.
Original language | English (US) |
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Pages (from-to) | 271-277 |
Number of pages | 7 |
Journal | Archives of Biochemistry and Biophysics |
Volume | 141 |
Issue number | 1 |
DOIs | |
State | Published - Nov 1970 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology