Studies of cloned simian immunodeficiency virus-specific T lymphocytes

gag-specific cytotoxic T lymphocytes exhibit a restricted epitope specificity

Hiroshi Yamamoto, Michael D. Miller, Hiroshi Tsubota, David Watkins, Gail P. Mazzara, Virginia Stallard, Dennis L. Panicali, Anna Aldovini, Richard A. Young, Norman L. Letvin

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

CD8+ CTL inhibit the replication of HIV and simian immunodeficiency virus of macaques (SIV mac) in PBL and, therefore, are likely to play an important role in containing the spread of the AIDS virus in infected individuals. We have generated a series of gag-specific lytic T lymphocyte clones from PBL: of an SIVmac-infected rhesus monkey. These T cell clones are CD3+CD8+ and are MHC class I-restricted in their target specificity. They are, therefore, CTL. Interestingly, all gag-specific CTL clones, as well as the gag-specific lytic activity of PBL of this monkey, demonstrated specificity for a single 25 amino acid fragment of the SIVmac gag protein. Moreover, they were restricted in their lytic function by a single MHC class I allele. These findings illustrate a powerful method for cloning AIDS virus-specific T lymphocytes and demonstrate a remarkably restricted epitope specificity of this AIDS virus-specific CTL response.

Original languageEnglish
Pages (from-to)3385-3391
Number of pages7
JournalJournal of Immunology
Volume144
Issue number9
StatePublished - May 1 1990
Externally publishedYes

Fingerprint

Simian Immunodeficiency Virus
Cytotoxic T-Lymphocytes
Epitopes
HIV
T-Lymphocytes
Clone Cells
gag Gene Products
Macaca
Macaca mulatta
Haplorhini
Organism Cloning
Alleles
Amino Acids

ASJC Scopus subject areas

  • Immunology

Cite this

Studies of cloned simian immunodeficiency virus-specific T lymphocytes : gag-specific cytotoxic T lymphocytes exhibit a restricted epitope specificity. / Yamamoto, Hiroshi; Miller, Michael D.; Tsubota, Hiroshi; Watkins, David; Mazzara, Gail P.; Stallard, Virginia; Panicali, Dennis L.; Aldovini, Anna; Young, Richard A.; Letvin, Norman L.

In: Journal of Immunology, Vol. 144, No. 9, 01.05.1990, p. 3385-3391.

Research output: Contribution to journalArticle

Yamamoto, H, Miller, MD, Tsubota, H, Watkins, D, Mazzara, GP, Stallard, V, Panicali, DL, Aldovini, A, Young, RA & Letvin, NL 1990, 'Studies of cloned simian immunodeficiency virus-specific T lymphocytes: gag-specific cytotoxic T lymphocytes exhibit a restricted epitope specificity', Journal of Immunology, vol. 144, no. 9, pp. 3385-3391.
Yamamoto, Hiroshi ; Miller, Michael D. ; Tsubota, Hiroshi ; Watkins, David ; Mazzara, Gail P. ; Stallard, Virginia ; Panicali, Dennis L. ; Aldovini, Anna ; Young, Richard A. ; Letvin, Norman L. / Studies of cloned simian immunodeficiency virus-specific T lymphocytes : gag-specific cytotoxic T lymphocytes exhibit a restricted epitope specificity. In: Journal of Immunology. 1990 ; Vol. 144, No. 9. pp. 3385-3391.
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abstract = "CD8+ CTL inhibit the replication of HIV and simian immunodeficiency virus of macaques (SIV mac) in PBL and, therefore, are likely to play an important role in containing the spread of the AIDS virus in infected individuals. We have generated a series of gag-specific lytic T lymphocyte clones from PBL: of an SIVmac-infected rhesus monkey. These T cell clones are CD3+CD8+ and are MHC class I-restricted in their target specificity. They are, therefore, CTL. Interestingly, all gag-specific CTL clones, as well as the gag-specific lytic activity of PBL of this monkey, demonstrated specificity for a single 25 amino acid fragment of the SIVmac gag protein. Moreover, they were restricted in their lytic function by a single MHC class I allele. These findings illustrate a powerful method for cloning AIDS virus-specific T lymphocytes and demonstrate a remarkably restricted epitope specificity of this AIDS virus-specific CTL response.",
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