Structure of the Yersinia pestis tip protein LcrV refined to 1.65 Å resolution

Sukanya Chaudhury, Kevin P. Battaile, Scott Lovell, Gregory V Plano, Roberto N. De Guzman

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The human pathogen Yersinia pestis requires the assembly of the type III secretion system (T3SS) for virulence. The structural component of the T3SS contains an external needle and a tip complex, which is formed by LcrV in Y. pestis. The structure of an LcrV triple mutant (K40A/D41A/K42A) in a C273S background has previously been reported to 2.2 Å resolution. Here, the crystal structure of LcrV without the triple mutation in a C273S background is reported at a higher resolution of 1.65 Å. Overall the two structures are similar, but there are also notable differences, particularly near the site of the triple mutation. The refined structure revealed a slight shift in the backbone positions of residues Gly28-Asn43 and displayed electron density in the loop region consisting of residues Ile46-Val63, which was disordered in the original structure. In addition, the helical turn region spanning residues Tyr77-Gln95 adopts a different orientation.

Original languageEnglish
Pages (from-to)477-481
Number of pages5
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume69
Issue number5
DOIs
StatePublished - May 1 2013

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Yersinia pestis
Pathogens
Needles
Carrier concentration
Crystal structure
mutations
proteins
Mutation
virulence
Virulence
secretions
Proteins
pathogens
Electrons
needles
assembly
crystal structure
shift
high resolution
Type III Secretion Systems

Keywords

  • LcrV
  • tip proteins
  • type III secretion system
  • Yersinia pestis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Structural Biology
  • Genetics
  • Condensed Matter Physics

Cite this

Structure of the Yersinia pestis tip protein LcrV refined to 1.65 Å resolution. / Chaudhury, Sukanya; Battaile, Kevin P.; Lovell, Scott; Plano, Gregory V; De Guzman, Roberto N.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 69, No. 5, 01.05.2013, p. 477-481.

Research output: Contribution to journalArticle

Chaudhury, Sukanya ; Battaile, Kevin P. ; Lovell, Scott ; Plano, Gregory V ; De Guzman, Roberto N. / Structure of the Yersinia pestis tip protein LcrV refined to 1.65 Å resolution. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2013 ; Vol. 69, No. 5. pp. 477-481.
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