Structure of the porcine LH- and FSH-releasing hormone. II. Confirmation of the proposed structure by conventional sequential analyses

Yoshihiko Baba, Hisayuki Matsuo, Andrew V. Schally

Research output: Contribution to journalArticle

190 Scopus citations

Abstract

The proposed amino acid sequence of porcine LH- and FSH-releasing hormone (LH-RH/FSH-RH) was reinvestigated by Edman-dansyl degradation after the cleavage of N-terminal pyroglutamyl residue by pyrrolidonecarboxylyl (PCA) peptidase. A C-terminal fragment from chymotrypic digest of LH-RH/FSH-RH was found to be identical to synthetic Gly-Leu-Arg-Pro-Gly-NH2. The results indicate that the structure initially proposed is correct. The amino acid sequence of porcine LH-RH/FSH-RH is thus (pyro)Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2.

Original languageEnglish (US)
Pages (from-to)459-463
Number of pages5
JournalBiochemical and biophysical research communications
Volume44
Issue number2
DOIs
StatePublished - Jul 16 1971
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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