Structure of the porcine LH- and FSH-releasing hormone. II. Confirmation of the proposed structure by conventional sequential analyses

Yoshihiko Baba; Hisayuki Matsuo; Andrew V. Schally

doi:10.1016/0006-291X(71)90623-1

Structure of the porcine LH- and FSH-releasing hormone. II. Confirmation of the proposed structure by conventional sequential analyses

Yoshihiko Baba, Hisayuki Matsuo, Andrew V. Schally

Research output: Contribution to journal › Article › peer-review

200 Scopus citations

Abstract

The proposed amino acid sequence of porcine LH- and FSH-releasing hormone (LH-RH/FSH-RH) was reinvestigated by Edman-dansyl degradation after the cleavage of N-terminal pyroglutamyl residue by pyrrolidonecarboxylyl (PCA) peptidase. A C-terminal fragment from chymotrypic digest of LH-RH/FSH-RH was found to be identical to synthetic Gly-Leu-Arg-Pro-Gly-NH₂. The results indicate that the structure initially proposed is correct. The amino acid sequence of porcine LH-RH/FSH-RH is thus (pyro)Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH₂.

Original language	English (US)
Pages (from-to)	459-463
Number of pages	5
Journal	Biochemical and biophysical research communications
Volume	44
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(71)90623-1
State	Published - Jul 16 1971
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(71)90623-1

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title = "Structure of the porcine LH- and FSH-releasing hormone. II. Confirmation of the proposed structure by conventional sequential analyses",

abstract = "The proposed amino acid sequence of porcine LH- and FSH-releasing hormone (LH-RH/FSH-RH) was reinvestigated by Edman-dansyl degradation after the cleavage of N-terminal pyroglutamyl residue by pyrrolidonecarboxylyl (PCA) peptidase. A C-terminal fragment from chymotrypic digest of LH-RH/FSH-RH was found to be identical to synthetic Gly-Leu-Arg-Pro-Gly-NH2. The results indicate that the structure initially proposed is correct. The amino acid sequence of porcine LH-RH/FSH-RH is thus (pyro)Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2.",

author = "Yoshihiko Baba and Hisayuki Matsuo and Schally, {Andrew V.}",

note = "Funding Information: * Supported by gmnts from VA, USPHS gmnt AM-07467 New York, N-Y, to AVS.",

year = "1971",

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doi = "10.1016/0006-291X(71)90623-1",

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T1 - Structure of the porcine LH- and FSH-releasing hormone. II. Confirmation of the proposed structure by conventional sequential analyses

AU - Baba, Yoshihiko

AU - Matsuo, Hisayuki

AU - Schally, Andrew V.

N1 - Funding Information: * Supported by gmnts from VA, USPHS gmnt AM-07467 New York, N-Y, to AVS.

PY - 1971/7/16

Y1 - 1971/7/16

N2 - The proposed amino acid sequence of porcine LH- and FSH-releasing hormone (LH-RH/FSH-RH) was reinvestigated by Edman-dansyl degradation after the cleavage of N-terminal pyroglutamyl residue by pyrrolidonecarboxylyl (PCA) peptidase. A C-terminal fragment from chymotrypic digest of LH-RH/FSH-RH was found to be identical to synthetic Gly-Leu-Arg-Pro-Gly-NH2. The results indicate that the structure initially proposed is correct. The amino acid sequence of porcine LH-RH/FSH-RH is thus (pyro)Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2.

AB - The proposed amino acid sequence of porcine LH- and FSH-releasing hormone (LH-RH/FSH-RH) was reinvestigated by Edman-dansyl degradation after the cleavage of N-terminal pyroglutamyl residue by pyrrolidonecarboxylyl (PCA) peptidase. A C-terminal fragment from chymotrypic digest of LH-RH/FSH-RH was found to be identical to synthetic Gly-Leu-Arg-Pro-Gly-NH2. The results indicate that the structure initially proposed is correct. The amino acid sequence of porcine LH-RH/FSH-RH is thus (pyro)Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2.

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JO - Biochemical and Biophysical Research Communications

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Structure of the porcine LH- and FSH-releasing hormone. II. Confirmation of the proposed structure by conventional sequential analyses

Abstract

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