The proposed amino acid sequence of porcine LH- and FSH-releasing hormone (LH-RH/FSH-RH) was reinvestigated by Edman-dansyl degradation after the cleavage of N-terminal pyroglutamyl residue by pyrrolidonecarboxylyl (PCA) peptidase. A C-terminal fragment from chymotrypic digest of LH-RH/FSH-RH was found to be identical to synthetic Gly-Leu-Arg-Pro-Gly-NH2. The results indicate that the structure initially proposed is correct. The amino acid sequence of porcine LH-RH/FSH-RH is thus (pyro)Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2.
|Original language||English (US)|
|Number of pages||5|
|Journal||Biochemical and biophysical research communications|
|State||Published - Jul 16 1971|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology