The complete amino acid sequence of porcine LH- and FSH- releasing hormone has been provisionally determined by the use on a micro-scale of the combined Edman-dansyl procedure coupled with the selective tritiation method for C-terminal analysis. These procedures were used directly on the digestion products of LH-RH with chymotrypsin and thermolysin, without separation of the fragments. Additional data were provided by high resolution mass spectral fragmentation of LH-RH. On the basis of these results, we propose the following decapeptide sequence for LH-RH: (pyro)Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and biophysical research communications|
|State||Published - Jun 18 1971|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology