Structure of the porcine LH- and FSH-releasing hormone. I. The proposed amino acid sequence

H. Matsuo, Y. Baba, R. M G Nair, A. Arimura, Andrew V Schally

Research output: Contribution to journalArticle

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Abstract

The complete amino acid sequence of porcine LH- and FSH- releasing hormone has been provisionally determined by the use on a micro-scale of the combined Edman-dansyl procedure coupled with the selective tritiation method for C-terminal analysis. These procedures were used directly on the digestion products of LH-RH with chymotrypsin and thermolysin, without separation of the fragments. Additional data were provided by high resolution mass spectral fragmentation of LH-RH. On the basis of these results, we propose the following decapeptide sequence for LH-RH: (pyro)Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2.

Original languageEnglish
Pages (from-to)1334-1339
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume43
Issue number6
DOIs
StatePublished - Jun 18 1971
Externally publishedYes

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Gonadotropin-Releasing Hormone
Amino Acid Sequence
Swine
Amino Acids
Thermolysin
Chymotrypsin
Digestion

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Structure of the porcine LH- and FSH-releasing hormone. I. The proposed amino acid sequence. / Matsuo, H.; Baba, Y.; Nair, R. M G; Arimura, A.; Schally, Andrew V.

In: Biochemical and Biophysical Research Communications, Vol. 43, No. 6, 18.06.1971, p. 1334-1339.

Research output: Contribution to journalArticle

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