Structure of a gap junction gene: Rat connexin-32

Todd Miller, Gerhard Dahl, Rudolf Werner

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

A genomic clone for the rat liver gap junction protein (connexin-32) was isolated and characterized by restriction enzyme mapping and sequence analysis. While the complete coding sequence is contained within one uninterrupted block, the 5′-untranslated region of the transcript contains a 6.1 kb intron. Both S1 nuclease protection and primer extension assays indicate multiple transcription start sites. Sequences homologous to cAMP response elements are found near the transcription start sites and within the 3′-end of the intron.

Original languageEnglish
Pages (from-to)455-464
Number of pages10
JournalBioscience Reports
Volume8
Issue number5
DOIs
StatePublished - Oct 1 1988

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Restriction Mapping
Transcription Initiation Site
Gap Junctions
Introns
Rats
Genes
Connexins
5' Untranslated Regions
Response Elements
Sequence Homology
Liver
Sequence Analysis
Assays
Clone Cells
Enzymes
connexin 32

Keywords

  • connexin
  • gap junction
  • gene structure

ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Structure of a gap junction gene : Rat connexin-32. / Miller, Todd; Dahl, Gerhard; Werner, Rudolf.

In: Bioscience Reports, Vol. 8, No. 5, 01.10.1988, p. 455-464.

Research output: Contribution to journalArticle

Miller, Todd ; Dahl, Gerhard ; Werner, Rudolf. / Structure of a gap junction gene : Rat connexin-32. In: Bioscience Reports. 1988 ; Vol. 8, No. 5. pp. 455-464.
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