Abstract
A genomic clone for the rat liver gap junction protein (connexin-32) was isolated and characterized by restriction enzyme mapping and sequence analysis. While the complete coding sequence is contained within one uninterrupted block, the 5′-untranslated region of the transcript contains a 6.1 kb intron. Both S1 nuclease protection and primer extension assays indicate multiple transcription start sites. Sequences homologous to cAMP response elements are found near the transcription start sites and within the 3′-end of the intron.
| Original language | English |
|---|---|
| Pages (from-to) | 455-464 |
| Number of pages | 10 |
| Journal | Bioscience Reports |
| Volume | 8 |
| Issue number | 5 |
| DOIs | |
| State | Published - Oct 1 1988 |
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Keywords
- connexin
- gap junction
- gene structure
ASJC Scopus subject areas
- Cell Biology
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Structure of a gap junction gene : Rat connexin-32. / Miller, Todd; Dahl, Gerhard; Werner, Rudolf.
In: Bioscience Reports, Vol. 8, No. 5, 01.10.1988, p. 455-464.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Structure of a gap junction gene
T2 - Rat connexin-32
AU - Miller, Todd
AU - Dahl, Gerhard
AU - Werner, Rudolf
PY - 1988/10/1
Y1 - 1988/10/1
N2 - A genomic clone for the rat liver gap junction protein (connexin-32) was isolated and characterized by restriction enzyme mapping and sequence analysis. While the complete coding sequence is contained within one uninterrupted block, the 5′-untranslated region of the transcript contains a 6.1 kb intron. Both S1 nuclease protection and primer extension assays indicate multiple transcription start sites. Sequences homologous to cAMP response elements are found near the transcription start sites and within the 3′-end of the intron.
AB - A genomic clone for the rat liver gap junction protein (connexin-32) was isolated and characterized by restriction enzyme mapping and sequence analysis. While the complete coding sequence is contained within one uninterrupted block, the 5′-untranslated region of the transcript contains a 6.1 kb intron. Both S1 nuclease protection and primer extension assays indicate multiple transcription start sites. Sequences homologous to cAMP response elements are found near the transcription start sites and within the 3′-end of the intron.
KW - connexin
KW - gap junction
KW - gene structure
UR - http://www.scopus.com/inward/record.url?scp=0024103248&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024103248&partnerID=8YFLogxK
U2 - 10.1007/BF01121644
DO - 10.1007/BF01121644
M3 - Article
C2 - 2852976
AN - SCOPUS:0024103248
VL - 8
SP - 455
EP - 464
JO - Bioscience Reports
JF - Bioscience Reports
SN - 0144-8463
IS - 5
ER -