Structure of a gap junction gene: Rat connexin-32

Todd Miller; Gerhard Dahl; Rudolf Werner

doi:10.1007/BF01121644

Structure of a gap junction gene: Rat connexin-32

Todd Miller, Gerhard Dahl, Rudolf Werner

Physiology & Biophysics

Research output: Contribution to journal › Article › peer-review

80 Scopus citations

Abstract

A genomic clone for the rat liver gap junction protein (connexin-32) was isolated and characterized by restriction enzyme mapping and sequence analysis. While the complete coding sequence is contained within one uninterrupted block, the 5′-untranslated region of the transcript contains a 6.1 kb intron. Both S1 nuclease protection and primer extension assays indicate multiple transcription start sites. Sequences homologous to cAMP response elements are found near the transcription start sites and within the 3′-end of the intron.

Original language	English (US)
Pages (from-to)	455-464
Number of pages	10
Journal	Bioscience reports
Volume	8
Issue number	5
DOIs	https://doi.org/10.1007/BF01121644
State	Published - Oct 1 1988

Keywords

connexin
gap junction
gene structure

ASJC Scopus subject areas

Cell Biology
Biochemistry, Genetics and Molecular Biology(all)

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10.1007/BF01121644

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abstract = "A genomic clone for the rat liver gap junction protein (connexin-32) was isolated and characterized by restriction enzyme mapping and sequence analysis. While the complete coding sequence is contained within one uninterrupted block, the 5′-untranslated region of the transcript contains a 6.1 kb intron. Both S1 nuclease protection and primer extension assays indicate multiple transcription start sites. Sequences homologous to cAMP response elements are found near the transcription start sites and within the 3′-end of the intron.",

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AB - A genomic clone for the rat liver gap junction protein (connexin-32) was isolated and characterized by restriction enzyme mapping and sequence analysis. While the complete coding sequence is contained within one uninterrupted block, the 5′-untranslated region of the transcript contains a 6.1 kb intron. Both S1 nuclease protection and primer extension assays indicate multiple transcription start sites. Sequences homologous to cAMP response elements are found near the transcription start sites and within the 3′-end of the intron.

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KW - gene structure

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Structure of a gap junction gene: Rat connexin-32

Abstract

Keywords

ASJC Scopus subject areas

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