Structure of a cloned circular Moloney murine leukemia virus DNA molecule containing an inverted segment

Implications for retrovirus integration

C. Shoemaker, S. Goff, Eli Gilboa, M. Paskind, S. W. Mitra, D. Baltimore

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

Closed circular Moloney murine leukemia virus (M-MuLV) DNA was prepared from recently infected cells and cloned in a λ vector. Four classes of cloned M-MuLV inserts were found: Class I, full length 8.8-kilobase (kb) inserts with two tandem long terminal repeats (LTRs) of 600 base pairs; class 2, 8.2-kb inserts with a single copy of a LTR; class 3, M-MuLV DNA inserts with various portions deleted; and class 4, an 8.8-kb insert with an internal sequence inversion. Determination of nucleotide sequence at the junction between the two LTRs from a class 1 insert suggested that circularization occurred by blunt-end ligation of an 8.8-kb linear DNA. The class 4 molecule had an inversion that was flanked by inverted LTRs, each of which had lost two terminal base pairs at the inversion end points. Also, four base pairs that were present only once in standard M-MuLV DNA were duplicated at either end of the inversion. This molecule was interpreted as resulting from an integrative inversion in which M-MuLV DNA has integrated into itself. Its analysis thus provided explicit information concerning the mechanism by which retrovirus DNA integrates into host cell DNA. Models of retrovirus integration based on bacterial DNA transposition mechanisms are proposed.

Original languageEnglish
Pages (from-to)3932-3936
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume77
Issue number7 II
DOIs
StatePublished - Dec 1 1980
Externally publishedYes

Fingerprint

Moloney murine leukemia virus
Retroviridae
Terminal Repeat Sequences
DNA
Base Pairing
Sequence Inversion
Bacterial DNA
Ligation

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Structure of a cloned circular Moloney murine leukemia virus DNA molecule containing an inverted segment : Implications for retrovirus integration. / Shoemaker, C.; Goff, S.; Gilboa, Eli; Paskind, M.; Mitra, S. W.; Baltimore, D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 77, No. 7 II, 01.12.1980, p. 3932-3936.

Research output: Contribution to journalArticle

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