Structure and expression of a gene encoding human calcitonin and calcitonin gene related peptide

Barry D. Nelkin, Kenneth I. Rosenfeld, Andrée de Bustros, Susan S. Leong, Bernard A. Roos, Stephen B. Baylin

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Messenger RNAs for calcitonin (CT) and calcitonin gene related peptide (CGRP) have been detected in a human medullary thyroid carcinoma cell line. DNA sequences of their cloned cDNAs, and genomic restriction mapping, indicate that both mRNAs probably originate from a single gene; the separate mRNAs are derived by alternative processing. The calcitonin gene is expressed in 10 of 10 examined culture lines of human lung cancer; most of these lines express a higher ratio of CGRP to CT specific mRNA than does the medullary thyroid carcinoma cell line.

Original languageEnglish
Pages (from-to)648-655
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume123
Issue number2
DOIs
StatePublished - Sep 17 1984
Externally publishedYes

Fingerprint

Gene encoding
Calcitonin Gene-Related Peptide
Calcitonin
Gene Expression
Messenger RNA
Genes
Cells
Cell Line
Restriction Mapping
DNA sequences
Cell culture
Lung Neoplasms
Complementary DNA
Processing
Medullary Thyroid cancer

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Structure and expression of a gene encoding human calcitonin and calcitonin gene related peptide. / Nelkin, Barry D.; Rosenfeld, Kenneth I.; de Bustros, Andrée; Leong, Susan S.; Roos, Bernard A.; Baylin, Stephen B.

In: Biochemical and Biophysical Research Communications, Vol. 123, No. 2, 17.09.1984, p. 648-655.

Research output: Contribution to journalArticle

Nelkin, Barry D. ; Rosenfeld, Kenneth I. ; de Bustros, Andrée ; Leong, Susan S. ; Roos, Bernard A. ; Baylin, Stephen B. / Structure and expression of a gene encoding human calcitonin and calcitonin gene related peptide. In: Biochemical and Biophysical Research Communications. 1984 ; Vol. 123, No. 2. pp. 648-655.
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