Structure and DNA sequence of the mouse MnSOD gene

D. DiSilvestre, S. R. Kleeberger, J. Johns, Roy C Levitt

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Oxidative damage to the cell has been implicated in the pathogenesis of a number of disorders, including chronic inflammation, aging, and cancer. Manganese superoxide dismutase (Mn-SOD) plays a major role in the protection of the mitochondrion from oxidative damage due to superoxide radicals and other excited oxygen species. In this report we describe the genomic organization and DNA sequence of the murine MnSOD gene. This gene is interrupted by four introns. The coding sequence of this gene was examined in C57BL/6J and C3H/HeJ mice that are susceptible and resistant, respectively, to the pulmonary injuries induced by the inhaled oxidants, ozone, and hyperoxia. Since the predicted amino acid sequence for MnSOD does not differ for these strains, nor does the size or steady-state level of this transcript, biologic variability in the pulmonary inflammatory response to ozone and hyperoxia does not arise from an altered gene structure. Examination of the noncoding sequence revealed a dC·dA polymorphism in intron 2 and a Styl RFLV in intron 4 of the MnSOD gene. These sequence and mapping data provide the basis for continued study of biologic variability in the MnSOD gene as a cause of disease.

Original languageEnglish
Pages (from-to)281-284
Number of pages4
JournalMammalian Genome
Volume6
Issue number4
DOIs
StatePublished - Apr 1 1995
Externally publishedYes

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Introns
Genes
Hyperoxia
Ozone
Inbred C3H Mouse
Lung Injury
Oxidants
Superoxides
Superoxide Dismutase
Amino Acid Sequence
Mitochondria
Oxygen
Inflammation
Lung
Neoplasms

ASJC Scopus subject areas

  • Genetics

Cite this

Structure and DNA sequence of the mouse MnSOD gene. / DiSilvestre, D.; Kleeberger, S. R.; Johns, J.; Levitt, Roy C.

In: Mammalian Genome, Vol. 6, No. 4, 01.04.1995, p. 281-284.

Research output: Contribution to journalArticle

DiSilvestre, D, Kleeberger, SR, Johns, J & Levitt, RC 1995, 'Structure and DNA sequence of the mouse MnSOD gene', Mammalian Genome, vol. 6, no. 4, pp. 281-284. https://doi.org/10.1007/BF00352417
DiSilvestre, D. ; Kleeberger, S. R. ; Johns, J. ; Levitt, Roy C. / Structure and DNA sequence of the mouse MnSOD gene. In: Mammalian Genome. 1995 ; Vol. 6, No. 4. pp. 281-284.
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