Structural phenotyping of stem cell-derived cardiomyocytes

Francesco Silvio Pasqualini, Sean Paul Sheehy, Ashutosh Agarwal, Yvonne Aratyn-Schaus, Kevin Kit Parker

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Structural phenotyping based on classical image feature detection has been adopted to elucidate the molecular mechanisms behind genetically or pharmacologically induced changes in cell morphology. Here, we developed a set of 11 metrics to capture the increasing sarcomere organization that occurs intracellularly during striated muscle cell development. To test our metrics, we analyzed the localization of the contractile protein α-actinin in a variety of primary and stem-cell derived cardiomyocytes. Further, we combined these metrics with data mining algorithms to unbiasedly score the phenotypic maturity of human-induced pluripotent stem cell-derived cardiomyocytes.

Original languageEnglish (US)
Pages (from-to)340-347
Number of pages8
JournalStem Cell Reports
Volume4
Issue number3
DOIs
StatePublished - Mar 10 2015
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Developmental Biology
  • Genetics

Cite this

Pasqualini, F. S., Sheehy, S. P., Agarwal, A., Aratyn-Schaus, Y., & Parker, K. K. (2015). Structural phenotyping of stem cell-derived cardiomyocytes. Stem Cell Reports, 4(3), 340-347. https://doi.org/10.1016/j.stemcr.2015.01.020