Structural investigation of cytochrome f Langmuir-Blodgett films with scanning tunneling microscopy: Protein aggregation

A. Tazi, S. Boussaad, J. A. Derose, R. M. Leblanc

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

We have imaged Langmuir-Blodgett (LB) films of cytochrome f (Cyt f), transferred onto a gold film on mica substrate which has been covered with arachidic acid (three monolayers), with the scanning tunneling microscope. From these images, we have determined the structure of the Cyt f LB films (two monolayers) as a function of surface pressure (II) and ethanol concentration (ethanol:water) in the spreading solvent. We have observed aggregation of the Cyt f protein in films deposited at 15 and 20 mN/m with ethanol concentrations of 20%, 40%, and 80% in the spreading solvent. The LB films of Cyt f are mainly composed of aggregates a few hundred nm in size. Film morphology and aggregate sizes depend upon the ethanol concentration and the surface pressure for transfer onto the solid substrate.

Original languageEnglish (US)
Pages (from-to)1476-1480
Number of pages5
JournalJournal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures
Volume14
Issue number2
StatePublished - Mar 1 1996

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Electrical and Electronic Engineering

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