Structural investigation of cytochrome f Langmuir-Blodgett films with scanning tunneling microscopy: Protein aggregation

A. Tazi, S. Boussaad, J. A. Derose, Roger Leblanc

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have imaged Langmuir-Blodgett (LB) films of cytochrome f (Cyt f), transferred onto a gold film on mica substrate which has been covered with arachidic acid (three monolayers), with the scanning tunneling microscope. From these images, we have determined the structure of the Cyt f LB films (two monolayers) as a function of surface pressure (II) and ethanol concentration (ethanol:water) in the spreading solvent. We have observed aggregation of the Cyt f protein in films deposited at 15 and 20 mN/m with ethanol concentrations of 20%, 40%, and 80% in the spreading solvent. The LB films of Cyt f are mainly composed of aggregates a few hundred nm in size. Film morphology and aggregate sizes depend upon the ethanol concentration and the surface pressure for transfer onto the solid substrate.

Original languageEnglish
Pages (from-to)1476-1480
Number of pages5
JournalJournal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures
Volume14
Issue number2
StatePublished - Mar 1 1996

Fingerprint

Langmuir Blodgett films
cytochromes
Scanning tunneling microscopy
Langmuir-Blodgett films
scanning tunneling microscopy
ethyl alcohol
Agglomeration
proteins
Proteins
Ethanol
Monolayers
mica
Mica
Substrates
microscopes
gold
Microscopes
Gold
acids
scanning

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Physics and Astronomy (miscellaneous)
  • Surfaces and Interfaces

Cite this

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abstract = "We have imaged Langmuir-Blodgett (LB) films of cytochrome f (Cyt f), transferred onto a gold film on mica substrate which has been covered with arachidic acid (three monolayers), with the scanning tunneling microscope. From these images, we have determined the structure of the Cyt f LB films (two monolayers) as a function of surface pressure (II) and ethanol concentration (ethanol:water) in the spreading solvent. We have observed aggregation of the Cyt f protein in films deposited at 15 and 20 mN/m with ethanol concentrations of 20{\%}, 40{\%}, and 80{\%} in the spreading solvent. The LB films of Cyt f are mainly composed of aggregates a few hundred nm in size. Film morphology and aggregate sizes depend upon the ethanol concentration and the surface pressure for transfer onto the solid substrate.",
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T1 - Structural investigation of cytochrome f Langmuir-Blodgett films with scanning tunneling microscopy

T2 - Protein aggregation

AU - Tazi, A.

AU - Boussaad, S.

AU - Derose, J. A.

AU - Leblanc, Roger

PY - 1996/3/1

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N2 - We have imaged Langmuir-Blodgett (LB) films of cytochrome f (Cyt f), transferred onto a gold film on mica substrate which has been covered with arachidic acid (three monolayers), with the scanning tunneling microscope. From these images, we have determined the structure of the Cyt f LB films (two monolayers) as a function of surface pressure (II) and ethanol concentration (ethanol:water) in the spreading solvent. We have observed aggregation of the Cyt f protein in films deposited at 15 and 20 mN/m with ethanol concentrations of 20%, 40%, and 80% in the spreading solvent. The LB films of Cyt f are mainly composed of aggregates a few hundred nm in size. Film morphology and aggregate sizes depend upon the ethanol concentration and the surface pressure for transfer onto the solid substrate.

AB - We have imaged Langmuir-Blodgett (LB) films of cytochrome f (Cyt f), transferred onto a gold film on mica substrate which has been covered with arachidic acid (three monolayers), with the scanning tunneling microscope. From these images, we have determined the structure of the Cyt f LB films (two monolayers) as a function of surface pressure (II) and ethanol concentration (ethanol:water) in the spreading solvent. We have observed aggregation of the Cyt f protein in films deposited at 15 and 20 mN/m with ethanol concentrations of 20%, 40%, and 80% in the spreading solvent. The LB films of Cyt f are mainly composed of aggregates a few hundred nm in size. Film morphology and aggregate sizes depend upon the ethanol concentration and the surface pressure for transfer onto the solid substrate.

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