Structural characterisation of Pinus radiata MADS-box DNA sequences isolated by PCR cloning

Daniel Yunqiu Wang, Rosie E. Bradshaw, Christian Walter, Marie B. Connett, David W. Fountain

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Flowering in all plant species analysed to date is regulated by highly conserved and developmentally regulated genes. Some of them were found to belong to the group of MADS-box genes. In the research described here, the construction of two opposing degenerate primers, targeted to highly conserved regions within the MADS box, allowed the amplification of a 78 bp segment from genomic DNA of Pinus radiata D.Don. By subcloning these PCR (Polymerase Chain Reaction) products into M13 and analysing the sequence of individual subclones, three different DNA sequences, each representing a conserved MADS-box region of three independent P. radiata genes were identified. The three MADS-box sequences shared nucleotide and amino acid sequence identity with homeotic genes of Arabidopsis thaliana: - two of them with the stamen-carpel-specific AGAMOUS gene and one with the stamen-petal-specific AP3 gene. The sequences isolated here are currently being used to isolate and characterise full-length MADS-box gene sequences which could provide a useful tool for the manipulation of flowering in P. radiata.

Original languageEnglish (US)
Pages (from-to)3-10
Number of pages8
JournalNew Zealand Journal of Forestry Science
Volume27
Issue number1
StatePublished - Dec 1 1997

Keywords

  • AGAMOUS-like
  • AP3-like
  • Floral development
  • MADS-box sequences
  • Pinus radiata

ASJC Scopus subject areas

  • Forestry
  • Chemical Engineering(all)
  • Ecology
  • Plant Science

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    Wang, D. Y., Bradshaw, R. E., Walter, C., Connett, M. B., & Fountain, D. W. (1997). Structural characterisation of Pinus radiata MADS-box DNA sequences isolated by PCR cloning. New Zealand Journal of Forestry Science, 27(1), 3-10.