Structural basis of lysine-acetylated HIV-1 Tat recognition by PCAF bromodomain

Shiraz Mujtaba, Yan He, Lei Zeng, Amjad Farooq, Justin E. Carlson, Melanie Ott, Eric Verdin, Ming Ming Zhou

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192 Scopus citations


The human immunodeficiency virus type 1 (HIV-1) trans-activator protein Tat stimulates transcription of the integrated HIV-1 genome and promotes viral replication in infected cells. Tat transactivation activity is dependent on lysine acetylation and its association with nuclear histone acetyltransferases p300/CBP (CREB binding protein) and p300/CBP-associated factor (PCAF). Here, we show that the bromodomain of PCAF binds specifically to HIV-1 Tat acetylated at lysine 50 and that this interaction competes effectively against HIV-1 TAR RNA binding to the lysine-acetylated Tat. The three-dimensional solution structure of the PCAF bromodomain in complex with a lysine 50-acetylated Tat peptide together with biochemical analyses provides the structural basis for the specificity of this molecular recognition and reveals insights into the differences in ligand selectivity of bromodomains.

Original languageEnglish (US)
Pages (from-to)575-586
Number of pages12
JournalMolecular Cell
Issue number3
StatePublished - Jan 1 2002


ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Mujtaba, S., He, Y., Zeng, L., Farooq, A., Carlson, J. E., Ott, M., Verdin, E., & Zhou, M. M. (2002). Structural basis of lysine-acetylated HIV-1 Tat recognition by PCAF bromodomain. Molecular Cell, 9(3), 575-586.