Abstract
Erythrosin B (Red Dye No. 3) and Rose Bengal photosensitize the destruction of the Ca2+:Mg2+-ATPase pump protein in sarcoplasmic reticulum (SR) vesicles with respective quantum efficiencies of (1.53 ± 0.19) × 10-3 and (1.25 ± 0.18) × 10-3. Damage to vesicle function was assayed by measurements of increases in passive Ca2+ permeability. Rates of passive Ca2+ movement into the SR lumen were increased by dye photosensitization in proportion to radiation absorbed. Active Ca2+ transport into SR vesicles was blocked independent of radiation absorbed by Erythrosin B and Rose Bengal at free concentrations of 0.69 μM and 1.16 μM, respectively. The photochemical lability of the Ca2+ pump protein and alterations in passive and active Ca2+ transport may be dependent on the concentration of the dye in the membrane. The photosensitization results may have implications with respect to the suitability of Erythrosin B usage in vivo, since the brightness of our irradiation source is comparable to that of sunlight at 480 nm.
Original language | English (US) |
---|---|
Pages (from-to) | 313-325 |
Number of pages | 13 |
Journal | Chemico-Biological Interactions |
Volume | 41 |
Issue number | 3 |
DOIs | |
State | Published - Sep 1982 |
ASJC Scopus subject areas
- Toxicology