Strong and selective adsorption of lysozyme on graphene oxide

Shanghao Li, Jerome J. Mulloor, Lingyu Wang, Yiwen Ji, Catherine J. Mulloor, Miodrag Micic, Jhony Orbulescu, Roger Leblanc

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Biosensing methods and devices using graphene oxide (GO) have recently been explored for detection and quantification of specific biomolecules from body fluid samples, such as saliva, milk, urine, and serum. For a practical diagnostics application, any sensing system must show an absence of nonselective detection of abundant proteins in the fluid matrix. Because lysozyme is an abundant protein in these body fluids (e.g., around 21.4 and 7 μg/mL of lysozyme is found in human milk and saliva from healthy individuals, and more than 15 or even 100 μg/mL in patients suffering from leukemia, renal disease, and sarcoidosis), it may interfere with detections and quantification if it has strong interaction with GO. Therefore, one fundamental question that needs to be addressed before any development of GO based diagnostics method is how GO interacts with lysozyme. In this study, GO has demonstrated a strong interaction with lysozyme. This interaction is so strong that we are able to subsequently eliminate and separate lysozyme from aqueous solution onto the surface of GO. Furthermore, the strong electrostatic interaction also renders the selective adsorption of lysozyme on GO from a mixture of binary and ternary proteins. This selectivity is confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), fluorescence spectroscopy, and UV-vis absorption spectroscopy.

Original languageEnglish
Pages (from-to)5704-5712
Number of pages9
JournalACS Applied Materials and Interfaces
Volume6
Issue number8
DOIs
StatePublished - Apr 23 2014

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Graphite
Muramidase
Oxides
Graphene
Enzymes
Adsorption
Body fluids
Proteins
Fluorescence spectroscopy
Sodium dodecyl sulfate
Biomolecules
Coulomb interactions
Polyacrylates
Electrophoresis
Ultraviolet spectroscopy
Absorption spectroscopy
Sodium Dodecyl Sulfate
Gels
Fluids

Keywords

  • fluorescence quenching
  • graphene oxide
  • interaction
  • lysozyme
  • selective adsorption

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Strong and selective adsorption of lysozyme on graphene oxide. / Li, Shanghao; Mulloor, Jerome J.; Wang, Lingyu; Ji, Yiwen; Mulloor, Catherine J.; Micic, Miodrag; Orbulescu, Jhony; Leblanc, Roger.

In: ACS Applied Materials and Interfaces, Vol. 6, No. 8, 23.04.2014, p. 5704-5712.

Research output: Contribution to journalArticle

Li, S, Mulloor, JJ, Wang, L, Ji, Y, Mulloor, CJ, Micic, M, Orbulescu, J & Leblanc, R 2014, 'Strong and selective adsorption of lysozyme on graphene oxide', ACS Applied Materials and Interfaces, vol. 6, no. 8, pp. 5704-5712. https://doi.org/10.1021/am500254e
Li S, Mulloor JJ, Wang L, Ji Y, Mulloor CJ, Micic M et al. Strong and selective adsorption of lysozyme on graphene oxide. ACS Applied Materials and Interfaces. 2014 Apr 23;6(8):5704-5712. https://doi.org/10.1021/am500254e
Li, Shanghao ; Mulloor, Jerome J. ; Wang, Lingyu ; Ji, Yiwen ; Mulloor, Catherine J. ; Micic, Miodrag ; Orbulescu, Jhony ; Leblanc, Roger. / Strong and selective adsorption of lysozyme on graphene oxide. In: ACS Applied Materials and Interfaces. 2014 ; Vol. 6, No. 8. pp. 5704-5712.
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