Stromal-cell-derived factor (SDF) 1-alpha in combination with BMP-2 and TGF-β1 induces site-directed cell homing and osteogenic and chondrogenic differentiation for tissue engineering without the requirement for cell seeding

Harvey Chim, Erin Miller, Christy Gliniak, Eben Alsberg

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The clinical translation of tissue engineering approaches is limited by the requirement of a cell source. Cell guidance is a new concept that provides an alternative approach, obviating a requirement for an external cell source. This relies on site-specific homing and differentiation of the patient's own cells to an implanted scaffold through controlled delivery of cytokines. In this study, we used stromal-cell-derived factor 1-alpha (SDF-1α) in combination with bone morphogenic protein (BMP)-2 or transforming growth factor (TGF)-β1 to induce cell migration and osteogenic or chondrogenic differentiation, respectively, in implanted scaffolds in a rat model. A customized cytokine microdelivery apparatus was used to ensure the constant rate and concentration of cytokine delivery around the scaffold. The formation of osteoid or early cartilage was observed after 4 weeks in specimens treated with SDF-1α and either BMP-2 or TGF-β1. The density of cellular infiltrate and formation of differentiated tissue were lower in scaffolds treated only with BMP-2 or TGF-β1. Thus, controlled SDF-1α delivery induces cell migration into scaffolds and can result in enhanced osteogenesis and chondrogenesis when used in combination with differentiation cytokines for purposes of tissue engineering.

Original languageEnglish (US)
Pages (from-to)89-94
Number of pages6
JournalCell and Tissue Research
Volume350
Issue number1
DOIs
StatePublished - Oct 2012
Externally publishedYes

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Chemokine CXCL12
Transforming Growth Factors
Tissue Engineering
Cytokines
Bone and Bones
Cell Movement
Proteins
Chondrogenesis
Osteogenesis
Cartilage

Keywords

  • Bone
  • Cartilage
  • Rat (Sprague Dawley)
  • SDF-1
  • Stem cells
  • Tissue engineering

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Histology

Cite this

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