Abstract
The clinical translation of tissue engineering approaches is limited by the requirement of a cell source. Cell guidance is a new concept that provides an alternative approach, obviating a requirement for an external cell source. This relies on site-specific homing and differentiation of the patient's own cells to an implanted scaffold through controlled delivery of cytokines. In this study, we used stromal-cell-derived factor 1-alpha (SDF-1α) in combination with bone morphogenic protein (BMP)-2 or transforming growth factor (TGF)-β1 to induce cell migration and osteogenic or chondrogenic differentiation, respectively, in implanted scaffolds in a rat model. A customized cytokine microdelivery apparatus was used to ensure the constant rate and concentration of cytokine delivery around the scaffold. The formation of osteoid or early cartilage was observed after 4 weeks in specimens treated with SDF-1α and either BMP-2 or TGF-β1. The density of cellular infiltrate and formation of differentiated tissue were lower in scaffolds treated only with BMP-2 or TGF-β1. Thus, controlled SDF-1α delivery induces cell migration into scaffolds and can result in enhanced osteogenesis and chondrogenesis when used in combination with differentiation cytokines for purposes of tissue engineering.
Original language | English (US) |
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Pages (from-to) | 89-94 |
Number of pages | 6 |
Journal | Cell and Tissue Research |
Volume | 350 |
Issue number | 1 |
DOIs | |
State | Published - Oct 1 2012 |
Keywords
- Bone
- Cartilage
- Rat (Sprague Dawley)
- SDF-1
- Stem cells
- Tissue engineering
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Histology
- Cell Biology