Stress-induced modulation of NK activity during influenza viral infection: Role of glucocorticoids and opioids

Raymond J. Tseng, David A. Padgett, Firdaus Dhabhar, Harald Engler, John F. Sheridan

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Activation of the hypothalamic-pituitary-adrenal axis (HPA) and sympathetic nervous system by stress has been shown to modulate both innate and adaptive immunity during an experimental influenza A/PR8 viral infection. HPA activation alters levels of glucocorticoids (GC) and opioids which are associated with suppression of lymphoid cellularity and NK activity. These experiments were designed to investigate the role that stress-induced GC and opioids have in modulating NK activity during an influenza viral infection. C57BL/6 mice were treated daily with mifepristone (RU486), a GC receptor antagonist or naltrexone (NTX), a non-specific opioid receptor antagonist. Mice were infected intranasally with A/PR8 virus and underwent daily restraint stress (RST). Phenotypic analysis of splenic cell populations and NK cytotoxicity were assessed at 3 days post-infection. RST of infected mice significantly suppressed splenic CD3-DX5+ cellularity and NK cytolytic activity. RU486 administration fully restored splenic NK cellularity but not cytolytic activity. NTX administration restored NK cytolytic activity but not splenic NK cell number. A similar restoration in NK cytolytic activity was observed after administration of β-funaltrexamine (FNA), a μ-specific opioid receptor antagonist, but not the δ- or κ-specific opioid receptor antagonists naltrindole or nor-binaltorphimine, respectively. Co-administration of RU486 and NTX restored both NK cellularity and cytolytic activity. The restoration of RST-induced suppression of NK activity by RU486 and NTX or FNA suggests that glucocorticoids modulate NK cellularity and opioids that bind to the μ-opioid receptor modulate NK cytotoxicity during periods of stress and viral infection.

Original languageEnglish (US)
Pages (from-to)153-164
Number of pages12
JournalBrain, Behavior, and Immunity
Volume19
Issue number2
DOIs
StatePublished - Mar 1 2005
Externally publishedYes

Fingerprint

Naltrexone
Virus Diseases
Opioid Analgesics
Human Influenza
Glucocorticoids
Narcotic Antagonists
naltrindole
Natural Killer Cells
Mifepristone
Sympathetic Nervous System
Glucocorticoid Receptors
Opioid Receptors
Adaptive Immunity
Inbred C57BL Mouse
Innate Immunity
Cell Count
Viruses
Infection
Population

Keywords

  • CD3 DX5 natural killer cells
  • Neuroendocrine modulation
  • NK cytotoxicity
  • Psychoneuroimmunology

ASJC Scopus subject areas

  • Immunology
  • Endocrine and Autonomic Systems
  • Behavioral Neuroscience

Cite this

Stress-induced modulation of NK activity during influenza viral infection : Role of glucocorticoids and opioids. / Tseng, Raymond J.; Padgett, David A.; Dhabhar, Firdaus; Engler, Harald; Sheridan, John F.

In: Brain, Behavior, and Immunity, Vol. 19, No. 2, 01.03.2005, p. 153-164.

Research output: Contribution to journalArticle

Tseng, Raymond J. ; Padgett, David A. ; Dhabhar, Firdaus ; Engler, Harald ; Sheridan, John F. / Stress-induced modulation of NK activity during influenza viral infection : Role of glucocorticoids and opioids. In: Brain, Behavior, and Immunity. 2005 ; Vol. 19, No. 2. pp. 153-164.
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