Streptolysin O enhances keratinocyte migration and proliferation and promotes skin organ culture wound healing in vitro

Marjana Tomic-Canic, Stephen W. Mamber, Olivera Stojadinovic, Brian Lee, Nadezda Radoja, John McMichael

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


ML-05, a modified form of the hemolytic and cytotoxic bacterial toxin, streptolysin O, is currently being investigated as a treatment for collagen-related disorders such as scleroderma and fibrosis. Furthermore, ML-05 may be effective in promoting wound healing and alleviating the formation of hypertrophic scars and keloids. To investigate the effects of ML-05 on wound-healing processes, in vitro wound-healing scratch assays (using human primary epidermal keratinocytes and dermal fibroblasts) and a human skin organ culture wound model were utilized. ML-05 markedly enhanced keratinocyte migration and proliferation in wound scratch assays. ML-05 did not affect either proliferation or migration of dermal fibroblasts, indicating that ML-05's effects on cell migration/proliferation may be keratinocyte-specific. ML-05 was tested in a dose-dependent manner in a skin organ culture wound model using two different application methods: Through the culture media (dermal exposure) or direct topical treatment of the wound surface. ML-05 was found to accelerate wound healing as measured by reepithelialization, particularly after topical application. Therefore, ML-05 may have potential as a wound-healing agent that promotes reepithelialization through stimulation of keratinocyte migration and proliferation.

Original languageEnglish (US)
Pages (from-to)71-79
Number of pages9
JournalWound Repair and Regeneration
Issue number1
StatePublished - Jan 2007
Externally publishedYes

ASJC Scopus subject areas

  • Surgery
  • Dermatology


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