Streptolysin O enhances keratinocyte migration and proliferation and promotes skin organ culture wound healing in vitro

Marjana Tomic-Canic, Stephen W. Mamber, Olivera Stojadinovic, Brian Lee, Nadezda Radoja, John McMichael

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

ML-05, a modified form of the hemolytic and cytotoxic bacterial toxin, streptolysin O, is currently being investigated as a treatment for collagen-related disorders such as scleroderma and fibrosis. Furthermore, ML-05 may be effective in promoting wound healing and alleviating the formation of hypertrophic scars and keloids. To investigate the effects of ML-05 on wound-healing processes, in vitro wound-healing scratch assays (using human primary epidermal keratinocytes and dermal fibroblasts) and a human skin organ culture wound model were utilized. ML-05 markedly enhanced keratinocyte migration and proliferation in wound scratch assays. ML-05 did not affect either proliferation or migration of dermal fibroblasts, indicating that ML-05's effects on cell migration/proliferation may be keratinocyte-specific. ML-05 was tested in a dose-dependent manner in a skin organ culture wound model using two different application methods: Through the culture media (dermal exposure) or direct topical treatment of the wound surface. ML-05 was found to accelerate wound healing as measured by reepithelialization, particularly after topical application. Therefore, ML-05 may have potential as a wound-healing agent that promotes reepithelialization through stimulation of keratinocyte migration and proliferation.

Original languageEnglish
Pages (from-to)71-79
Number of pages9
JournalWound Repair and Regeneration
Volume15
Issue number1
DOIs
StatePublished - Jan 1 2007
Externally publishedYes

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Organ Culture Techniques
Keratinocytes
Wound Healing
Skin
Wounds and Injuries
Fibroblasts
Hypertrophic Cicatrix
Bacterial Toxins
Keloid
Cell Movement
Culture Media
Fibrosis
Collagen
Cell Proliferation
In Vitro Techniques
streptolysin O

ASJC Scopus subject areas

  • Dermatology
  • Surgery

Cite this

Streptolysin O enhances keratinocyte migration and proliferation and promotes skin organ culture wound healing in vitro. / Tomic-Canic, Marjana; Mamber, Stephen W.; Stojadinovic, Olivera; Lee, Brian; Radoja, Nadezda; McMichael, John.

In: Wound Repair and Regeneration, Vol. 15, No. 1, 01.01.2007, p. 71-79.

Research output: Contribution to journalArticle

Tomic-Canic, Marjana ; Mamber, Stephen W. ; Stojadinovic, Olivera ; Lee, Brian ; Radoja, Nadezda ; McMichael, John. / Streptolysin O enhances keratinocyte migration and proliferation and promotes skin organ culture wound healing in vitro. In: Wound Repair and Regeneration. 2007 ; Vol. 15, No. 1. pp. 71-79.
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