Stored calcium modulates inositol phosphate synthesis in cultured smooth muscle cells

Dora Berman-Weinberg, W. F. Goldman

Research output: Contribution to journal › Article

6 Citations (Scopus)

Abstract

Cytosolic Ca2+ concentrations ([Ca2+](cyt)) and [3H]inositol phosphates ([3H]InsP) were correlated while varying the Ca2+ content of the sarcoplasmic reticulum (SR) in cultured A7r5 cells at rest and during activation with [Arg8]-vasopressin (AVP). Thapsigargin (TG) raised and superfusion with 0 Ca2+ lowered [Ca2+](cyt), but both treatments decreased SR Ca2+ and AVP-evoked Ca2+ transients. Neither TG nor 0 Ca2+ affected basal [3H]InsP, but both treatments increased AVP-evoked synthesis of [3H]InsP. Exposure for several minutes to 40 mM K+ solution, BAY K 8644, or low-Na+ solution all elevated [Ca2+](cyt) and, thereby, increased SR Ca2+, as manifested by augmented AVP-evoked Ca2+ transients. In all three cases, AVP-evoked, but not basal, [3H]InsP were reduced. The inhibitory effect of 40 mM K+ on AVP-evoked [3H]InsP synthesis was blocked when SR Ca2+ uptake was prevented by TG. Brief (30-s) exposures to 40 mM K+, which elevated [Ca2+](cyt) but not SR Ca2+ loading, did not modify AVP-evoked [3H]InsP synthesis or Ca2+ transients. These results demonstrate an inverse relationship between SR Ca2+ content and evoked [3H]-InsP synthesis. Moreover, they suggest that SR Ca2+ may serve as a signal that modulates sarcolemmal [3H]InsP formation.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume263
Issue number2 32-2
StatePublished - Jan 1 1992
Externally publishedYes

Fingerprint

Inositol Phosphates
Sarcoplasmic Reticulum
Vasopressins
Smooth Muscle Myocytes
Muscle
Cells
Calcium
Thapsigargin
Cultured Cells
Chemical activation

Keywords

  • A7r5 cells
  • calcium release
  • fura-2
  • sarcoplasmic reticulum
  • thapsigargin
  • vasopressin

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Stored calcium modulates inositol phosphate synthesis in cultured smooth muscle cells. / Berman-Weinberg, Dora; Goldman, W. F.

In: American Journal of Physiology - Cell Physiology, Vol. 263, No. 2 32-2, 01.01.1992.

Research output: Contribution to journal › Article

Berman-Weinberg, D & Goldman, WF 1992, 'Stored calcium modulates inositol phosphate synthesis in cultured smooth muscle cells', American Journal of Physiology - Cell Physiology, vol. 263, no. 2 32-2.
Berman-Weinberg D, Goldman WF. Stored calcium modulates inositol phosphate synthesis in cultured smooth muscle cells. American Journal of Physiology - Cell Physiology. 1992 Jan 1;263(2 32-2).
Berman-Weinberg, Dora ; Goldman, W. F. / Stored calcium modulates inositol phosphate synthesis in cultured smooth muscle cells. In: American Journal of Physiology - Cell Physiology. 1992 ; Vol. 263, No. 2 32-2.
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