Stool phospholipid signature is altered by diet and tumors

Julie M. Davies, Hong Uyen Hua, Rishu Dheer, Mitchell Martinez, Sanjoy K Bhattacharya, Maria T Abreu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Intake of saturated fat is a risk factor for ulcerative colitis (UC) and colon cancer. Changes in the microbiota have been implicated in the development of UC and colon cancer. The host and the microbiota generate metabolites that may contribute to or reflect disease pathogenesis. We used lipid class specific quantitative mass spectrometry to assess the phospholipid (PL) profile (phosphatidylcholine [PC], phosphatidylethanolamine [PE], phosphatidylinositol [PI], phosphatidylserine [PS]) of stool from mice fed a high fat (HFD) or control diet with or without induction of colitis-associated tumors using azoxymethane and dextran sodium sulfate. The microbiota was assessed using qPCR for several bacterial groups. Colitisassociated tumors were associated with reduced bulk PI and PE levels in control diet fed mice compared to untreated mice. Significant decreases in the relative quantities of several PC species were found in colitis-associated tumor bearing mice fed either diet. Statistical analysis of the PL profile revealed distinct clustering by treatment group. Partial least squares regression analysis found that the relative quantities of the PS class profile best predicted bacterial abundance of Clostridium leptum and Prevotella groups. Abundance of selected PL species correlated with bacterial group quantities. Thus, we have described that a HFD and colitisassociated tumors are associated with changes in phospholipids and may reflect host-microbial interactions and disease states.

Original languageEnglish (US)
Article numbere114352
JournalPLoS One
Volume9
Issue number12
DOIs
StatePublished - Dec 1 2014

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colitis
Nutrition
Tumors
Microbiota
Phospholipids
phospholipids
Diet
neoplasms
phosphatidylserines
Phosphatidylserines
mice
phosphatidylinositols
Colitis
phosphatidylethanolamines
Phosphatidylinositols
colorectal neoplasms
Phosphatidylcholines
Ulcerative Colitis
diet
phosphatidylcholines

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Stool phospholipid signature is altered by diet and tumors. / Davies, Julie M.; Hua, Hong Uyen; Dheer, Rishu; Martinez, Mitchell; Bhattacharya, Sanjoy K; Abreu, Maria T.

In: PLoS One, Vol. 9, No. 12, e114352, 01.12.2014.

Research output: Contribution to journalArticle

Davies, Julie M. ; Hua, Hong Uyen ; Dheer, Rishu ; Martinez, Mitchell ; Bhattacharya, Sanjoy K ; Abreu, Maria T. / Stool phospholipid signature is altered by diet and tumors. In: PLoS One. 2014 ; Vol. 9, No. 12.
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