Stool phospholipid signature is altered by diet and tumors

Julie M. Davies, Hong Uyen Hua, Rishu Dheer, Mitchell Martinez, Sanjoy K. Bhattacharya, Maria T. Abreu

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Intake of saturated fat is a risk factor for ulcerative colitis (UC) and colon cancer. Changes in the microbiota have been implicated in the development of UC and colon cancer. The host and the microbiota generate metabolites that may contribute to or reflect disease pathogenesis. We used lipid class specific quantitative mass spectrometry to assess the phospholipid (PL) profile (phosphatidylcholine [PC], phosphatidylethanolamine [PE], phosphatidylinositol [PI], phosphatidylserine [PS]) of stool from mice fed a high fat (HFD) or control diet with or without induction of colitis-associated tumors using azoxymethane and dextran sodium sulfate. The microbiota was assessed using qPCR for several bacterial groups. Colitisassociated tumors were associated with reduced bulk PI and PE levels in control diet fed mice compared to untreated mice. Significant decreases in the relative quantities of several PC species were found in colitis-associated tumor bearing mice fed either diet. Statistical analysis of the PL profile revealed distinct clustering by treatment group. Partial least squares regression analysis found that the relative quantities of the PS class profile best predicted bacterial abundance of Clostridium leptum and Prevotella groups. Abundance of selected PL species correlated with bacterial group quantities. Thus, we have described that a HFD and colitisassociated tumors are associated with changes in phospholipids and may reflect host-microbial interactions and disease states.

Original languageEnglish (US)
Article numbere114352
JournalPloS one
Volume9
Issue number12
DOIs
StatePublished - Dec 2014

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Fingerprint Dive into the research topics of 'Stool phospholipid signature is altered by diet and tumors'. Together they form a unique fingerprint.

  • Cite this