Stimulation of Release and Synthesis of Growth Hormone (GH) in Tissue Cultures of Anterior Pituitaries in Response to GH-Releasing Hormone (GH-RH) (34588)

J. C. Mittler, S. Sawano, I. Wakabayashi, T. W. Redding, A. V. Schally

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Addition of small amounts of pure or highly purified porcine GH-RH twice daily to rat anterior pituitary tissue, maintained for 5 days in Trowell's T8 medium, significantly increased the amounts of GH in the medium and tissues relative to the controls as measured by bioassay. Apparent incorporation of radioactive amino acids into the GH bands on polyacrylamide gels after electrophoresis of medium and tissue was increased also. These results support other findings from this laboratory on the identity and properties of this neurohormone.

Original languageEnglish (US)
Pages (from-to)890-893
Number of pages4
JournalProceedings of the Society for Experimental Biology and Medicine
Volume133
Issue number3
DOIs
StatePublished - Mar 1970
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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