Addition of small amounts of pure or highly purified porcine GH-RH twice daily to rat anterior pituitary tissue, maintained for 5 days in Trowell's T8 medium, significantly increased the amounts of GH in the medium and tissues relative to the controls as measured by bioassay. Apparent incorporation of radioactive amino acids into the GH bands on polyacrylamide gels after electrophoresis of medium and tissue was increased also. These results support other findings from this laboratory on the identity and properties of this neurohormone.
|Original language||English (US)|
|Number of pages||4|
|Journal||Proceedings of the Society for Experimental Biology and Medicine|
|State||Published - Mar 1970|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)