Stimulation of Pancreatic Growth by Cholecystokinin Is Mediated by High-Affinity Receptors on Rat Pancreatic Acinar Cells

R. Dawra, A. Saluja, M. M. Lerch, M. Saluja, C. Logsdon, M. Steer

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Pancreatic acinar cells possess both high and low affinity receptors for cholecystokinin. The cholecystokinin analog caerulein, which exerts a trophic effect on the rat pancreas, acts as an agonist at both types of receptors. In contrast, the synthetic analog CCK-JMV-180, which also acts as an agonist at high affinity receptors, opposes the action of caerulein on the low affinity receptors. We report that infusion of either caerulein or CCK-JMV- 180 into rats increases [3H]-thymidine incorporation into pancreatic DNA and causes the pancreatic weight as well as content of DNA, RNA, and protein to increase. CCK-JMV-180 also stimulates in-vitro incorporation of [3H]-thymidine into DNA of cultured rat acini. The finding that both caerulein and CCK-JMV-180 exert the same trophic effect on pancreatic acinar cells indicates that this effect is mediated via high affinity acinar cell cholecystokinin receptors.

Original languageEnglish (US)
Pages (from-to)814-820
Number of pages7
JournalBiochemical and biophysical research communications
Volume193
Issue number3
DOIs
StatePublished - Jun 30 1993

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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