Stimulation of cyclic AMP accumulation and corticotropin release by synthetic ovine corticotropin-releasing factor in rat anterior pituitary cells: Site of glucocorticoid action

V. Giguere, F. Labrie, J. Cote, D. H. Coy, J. Sueiras-Diaz, Andrew V Schally

Research output: Contribution to journalArticle

151 Citations (Scopus)

Abstract

A 2.5-fold stimulation of cyclic AMP cellular content is measured 60 sec after addition of 100 nM synthetic ovine corticotropin-releasing factor (C-RF; corticoliberin) to rat anterior pituitary cells in culture. A maximal response of cyclic AMP content at 400% above control is observed between 2 and 30 min after addition of the peptide, whereas an 8-fold stimulation of cyclic AMP released into the incubation medium is measured between 10 and 180 min. A linear 7-fold increase of corticotropin release is observed for up to 3 hr. Preincubation for 18 hr with the potent glucocorticoid dexamethasone has no effect on C-RF-induced cyclic AMP accumulation. The same treatment with dexamethasone causes an 80% inhibition of corticotropin release induced by both C-RF and the cyclic AMP derivative 8-bromoadenosine 3',5'-cyclic monophosphate. The present data show that ovine C-RF is a potent stimulator of cyclic AMP accumulation in rat anterior pituitary cells and that the process is insensitive to the action of dexamethasone. The marked inhibition by dexamethasone of corticotropin secretion induced by a cyclic AMP derivative indicates that glucocorticoids exert their potent inhibitory effect on corticotropin secretion at a step distant to cyclic AMP formation.

Original languageEnglish
Pages (from-to)3466-3469
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume79
Issue number11 I
DOIs
StatePublished - Dec 1 1982
Externally publishedYes

Fingerprint

Corticotropin-Releasing Hormone
Cyclic AMP
Adrenocorticotropic Hormone
Glucocorticoids
Sheep
Dexamethasone
8-Bromo Cyclic Adenosine Monophosphate
Cell Culture Techniques
Peptides

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

@article{5ff51e8b14394c24b886b08b81f8e4e7,
title = "Stimulation of cyclic AMP accumulation and corticotropin release by synthetic ovine corticotropin-releasing factor in rat anterior pituitary cells: Site of glucocorticoid action",
abstract = "A 2.5-fold stimulation of cyclic AMP cellular content is measured 60 sec after addition of 100 nM synthetic ovine corticotropin-releasing factor (C-RF; corticoliberin) to rat anterior pituitary cells in culture. A maximal response of cyclic AMP content at 400{\%} above control is observed between 2 and 30 min after addition of the peptide, whereas an 8-fold stimulation of cyclic AMP released into the incubation medium is measured between 10 and 180 min. A linear 7-fold increase of corticotropin release is observed for up to 3 hr. Preincubation for 18 hr with the potent glucocorticoid dexamethasone has no effect on C-RF-induced cyclic AMP accumulation. The same treatment with dexamethasone causes an 80{\%} inhibition of corticotropin release induced by both C-RF and the cyclic AMP derivative 8-bromoadenosine 3',5'-cyclic monophosphate. The present data show that ovine C-RF is a potent stimulator of cyclic AMP accumulation in rat anterior pituitary cells and that the process is insensitive to the action of dexamethasone. The marked inhibition by dexamethasone of corticotropin secretion induced by a cyclic AMP derivative indicates that glucocorticoids exert their potent inhibitory effect on corticotropin secretion at a step distant to cyclic AMP formation.",
author = "V. Giguere and F. Labrie and J. Cote and Coy, {D. H.} and J. Sueiras-Diaz and Schally, {Andrew V}",
year = "1982",
month = "12",
day = "1",
doi = "10.1073/pnas.79.11.3466",
language = "English",
volume = "79",
pages = "3466--3469",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "11 I",

}

TY - JOUR

T1 - Stimulation of cyclic AMP accumulation and corticotropin release by synthetic ovine corticotropin-releasing factor in rat anterior pituitary cells

T2 - Site of glucocorticoid action

AU - Giguere, V.

AU - Labrie, F.

AU - Cote, J.

AU - Coy, D. H.

AU - Sueiras-Diaz, J.

AU - Schally, Andrew V

PY - 1982/12/1

Y1 - 1982/12/1

N2 - A 2.5-fold stimulation of cyclic AMP cellular content is measured 60 sec after addition of 100 nM synthetic ovine corticotropin-releasing factor (C-RF; corticoliberin) to rat anterior pituitary cells in culture. A maximal response of cyclic AMP content at 400% above control is observed between 2 and 30 min after addition of the peptide, whereas an 8-fold stimulation of cyclic AMP released into the incubation medium is measured between 10 and 180 min. A linear 7-fold increase of corticotropin release is observed for up to 3 hr. Preincubation for 18 hr with the potent glucocorticoid dexamethasone has no effect on C-RF-induced cyclic AMP accumulation. The same treatment with dexamethasone causes an 80% inhibition of corticotropin release induced by both C-RF and the cyclic AMP derivative 8-bromoadenosine 3',5'-cyclic monophosphate. The present data show that ovine C-RF is a potent stimulator of cyclic AMP accumulation in rat anterior pituitary cells and that the process is insensitive to the action of dexamethasone. The marked inhibition by dexamethasone of corticotropin secretion induced by a cyclic AMP derivative indicates that glucocorticoids exert their potent inhibitory effect on corticotropin secretion at a step distant to cyclic AMP formation.

AB - A 2.5-fold stimulation of cyclic AMP cellular content is measured 60 sec after addition of 100 nM synthetic ovine corticotropin-releasing factor (C-RF; corticoliberin) to rat anterior pituitary cells in culture. A maximal response of cyclic AMP content at 400% above control is observed between 2 and 30 min after addition of the peptide, whereas an 8-fold stimulation of cyclic AMP released into the incubation medium is measured between 10 and 180 min. A linear 7-fold increase of corticotropin release is observed for up to 3 hr. Preincubation for 18 hr with the potent glucocorticoid dexamethasone has no effect on C-RF-induced cyclic AMP accumulation. The same treatment with dexamethasone causes an 80% inhibition of corticotropin release induced by both C-RF and the cyclic AMP derivative 8-bromoadenosine 3',5'-cyclic monophosphate. The present data show that ovine C-RF is a potent stimulator of cyclic AMP accumulation in rat anterior pituitary cells and that the process is insensitive to the action of dexamethasone. The marked inhibition by dexamethasone of corticotropin secretion induced by a cyclic AMP derivative indicates that glucocorticoids exert their potent inhibitory effect on corticotropin secretion at a step distant to cyclic AMP formation.

UR - http://www.scopus.com/inward/record.url?scp=0020264294&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020264294&partnerID=8YFLogxK

U2 - 10.1073/pnas.79.11.3466

DO - 10.1073/pnas.79.11.3466

M3 - Article

C2 - 6285350

AN - SCOPUS:0020264294

VL - 79

SP - 3466

EP - 3469

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 11 I

ER -