Steroid sensitivity of norepinephrine uptake by human bronchial arterial and rabbit aortic smooth muscle cells

G. Horvath, T. Lieb, Gregory E Conner, Matthias A Salathe, Adam Wanner

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We have shown that an inhaled glucocorticosteroid (GS) causes α1-adrenergic antagonist-blockable, rapid, and transient bronchial vasoconstriction in healthy and asthmatic subjects. Steroids inhibit norepinephrine (NE) uptake by non-neuronal cells, thereby increasing NE concentration at α-adrenergic receptor sites. This could explain the GS-induced bronchial vasoconstriction. We therefore studied expression of the steroid-sensitive extraneuronal monoamine transporter (EMT) and steroid sensitivity of NE uptake in human bronchial artery and rabbit aorta (as a substitute for the limited supply of human bronchial artery). NE uptake was measured using a semiquantitative, sucrose-potassium phosphate-glyoxylic acid fluorescence method that we newly adapted for use in single cells. Both human bronchial arteries and rabbit aorta expressed messenger RNA for EMT, and steroids blocked NE uptake into freshly dissociated human bronchial arterial and rabbit aortic smooth-muscle cells (SMCs). In the latter, inhibition of NE uptake by steroids was not altered, either by a protein synthesis inhibitor (cycloheximide) or by a transcription inhibitor (actinomycin D), and corticosterone made membrane-impermeant by conjugation to bovine serum albumin inhibited NE uptake equipotently. These data show that NE uptake into bronchial arterial and rabbit aortic SMCs is sensitive to steroids, possibly mediated by EMT, and suggest a mechanism for GS-induced bronchial vasoconstriction.

Original languageEnglish
Pages (from-to)500-506
Number of pages7
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume25
Issue number4
StatePublished - Nov 20 2001

Fingerprint

Smooth Muscle Myocytes
Muscle
Norepinephrine
Steroids
Cells
Rabbits
Bronchial Arteries
Vasoconstriction
Aorta
Protein Synthesis Inhibitors
Adrenergic Antagonists
Dactinomycin
Transcription
Cycloheximide
Bovine Serum Albumin
Corticosterone
Adrenergic Receptors
Sucrose
Healthy Volunteers
Fluorescence

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

Steroid sensitivity of norepinephrine uptake by human bronchial arterial and rabbit aortic smooth muscle cells. / Horvath, G.; Lieb, T.; Conner, Gregory E; Salathe, Matthias A; Wanner, Adam.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 25, No. 4, 20.11.2001, p. 500-506.

Research output: Contribution to journalArticle

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