Steroid hormone induction and expression patterns of L-plastin in normal and carcinomatous prostate tissues

Jianping Zheng, Nandini Rudra-Ganguly, Gary J. Miller, Kirsten A. Moffatt, Richard J Cote, Pradip Roy-Burman

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Application of differential display to the comparison of androgen- stimulated and unstimulated human prostate carcinoma cell line LNCaP identified androgen induction of the L-plastin gene, which encodes an actin- binding protein isoform. Further investigation demonstrated that L-plastin expression in LNCaP cells is up-regulated by both dihydrotestosterone and estradiol. This induction of expression is detected as early as 2 hours after addition of steroids to the cell culture. L-plastin expression is also detected in other prostate carcinoma cell lines by reverse transcriptase polymerase chain reaction and immunohistochemistry but not in the single normal adult prostate epithelial cell line that is available to us. Analysis of multiple primary prostatic tumor tissues as well as normal and tumor tissues of the same prostate gland showed that tumor tissues exhibit a higher level of expression as compared with the normal tissues. Immunohistochemical study using anti-L-plastin antiserum on normal and carcinomatous prostate tissues showed a very striking difference in the staining patterns. Positive staining was seen in the fibromuscular stroma in normal prostates but not in the glandular epithelial cells. In contrast, strong staining was seen predominantly within the carcinomatous glandular epithelial cells. Taken together, these results suggest that the expression of L-plastin in prostatic epithelial cells is linked to the malignant state and that, once expressed in carcinomas, its expression is regulated by steroid hormone receptors.

Original languageEnglish
Pages (from-to)2009-2018
Number of pages10
JournalAmerican Journal of Pathology
Volume150
Issue number6
StatePublished - Jun 16 1997
Externally publishedYes

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Prostate
Prostatic Neoplasms
Steroids
Hormones
Epithelial Cells
Staining and Labeling
Carcinoma
Cell Line
Androgens
Neoplasms
Microfilament Proteins
Dihydrotestosterone
Steroid Receptors
Reverse Transcriptase Polymerase Chain Reaction
Immune Sera
Estradiol
Protein Isoforms
Cell Culture Techniques
Immunohistochemistry
plastin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Zheng, J., Rudra-Ganguly, N., Miller, G. J., Moffatt, K. A., Cote, R. J., & Roy-Burman, P. (1997). Steroid hormone induction and expression patterns of L-plastin in normal and carcinomatous prostate tissues. American Journal of Pathology, 150(6), 2009-2018.

Steroid hormone induction and expression patterns of L-plastin in normal and carcinomatous prostate tissues. / Zheng, Jianping; Rudra-Ganguly, Nandini; Miller, Gary J.; Moffatt, Kirsten A.; Cote, Richard J; Roy-Burman, Pradip.

In: American Journal of Pathology, Vol. 150, No. 6, 16.06.1997, p. 2009-2018.

Research output: Contribution to journalArticle

Zheng, J, Rudra-Ganguly, N, Miller, GJ, Moffatt, KA, Cote, RJ & Roy-Burman, P 1997, 'Steroid hormone induction and expression patterns of L-plastin in normal and carcinomatous prostate tissues', American Journal of Pathology, vol. 150, no. 6, pp. 2009-2018.
Zheng, Jianping ; Rudra-Ganguly, Nandini ; Miller, Gary J. ; Moffatt, Kirsten A. ; Cote, Richard J ; Roy-Burman, Pradip. / Steroid hormone induction and expression patterns of L-plastin in normal and carcinomatous prostate tissues. In: American Journal of Pathology. 1997 ; Vol. 150, No. 6. pp. 2009-2018.
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