Stem Cells from Amniotic Fluid

Mara Cananzi, Anthony Atala, Paolo de Coppi

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

This chapter provides an overview of the potential advantages and disadvantages of different stem and progenitor cell populations identified to date in amniotic fluid, along with their properties and potential clinical applications. Two stem cell populations are isolated from the AF that include amniotic fluid mesenchymal stem cells (AFMSCs) and amniotic fluid stem (AFS) cells and both can be used as primary (not transformed or immortalized) cells without further technical manipulations. AFMSCs exhibit typical mesenchymal stromal cell (MSC) characteristics such as fibroblastic-like morphology, clonogenic capacity, multilineage differentiation potential, immunosuppressive properties, and expression of a mesenchymal gene expression profile and of a mesenchymal set of surface antigens. AFMSCs are easier to isolate and show better proliferation capacities. The harvest of bone marrow remains, in fact, a highly invasive and painful procedure, and the number, the proliferation, and the differentiation potential of these cells decline with increasing age. Similarly, UCB-derived MSCs exist at a low percentage and expand slowly in culture. AFS cells represent a novel class of pluripotent stem cells with intermediate characteristics between ES cells and AS cells. They express both embryonic and mesenchymal stem cell markers, are able to differentiate into lineages representative of all embryonic germ layers, and do not form tumors after implantation in vivo. AFS cells may not differentiate as promptly as ES cells and their lack of tumorigenesis can be argued against their pluripotency.

Original languageEnglish
Title of host publicationPrinciples of Regenerative Medicine
PublisherElsevier Inc.
Pages223-239
Number of pages17
ISBN (Print)9780123814227
DOIs
StatePublished - Dec 1 2011
Externally publishedYes

Fingerprint

Amniotic Fluid
Stem cells
Stem Cells
Mesenchymal Stromal Cells
Fluids
Germ Layers
Pluripotent Stem Cells
Surface Antigens
Immunosuppressive Agents
Embryonic Stem Cells
Transcriptome
Population
Cell Differentiation
Carcinogenesis
Cell culture
Bone Marrow
Gene expression
Tumors
Bone
Cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Cananzi, M., Atala, A., & Coppi, P. D. (2011). Stem Cells from Amniotic Fluid. In Principles of Regenerative Medicine (pp. 223-239). Elsevier Inc.. https://doi.org/10.1016/B978-0-12-381422-7.10012-4

Stem Cells from Amniotic Fluid. / Cananzi, Mara; Atala, Anthony; Coppi, Paolo de.

Principles of Regenerative Medicine. Elsevier Inc., 2011. p. 223-239.

Research output: Chapter in Book/Report/Conference proceedingChapter

Cananzi, M, Atala, A & Coppi, PD 2011, Stem Cells from Amniotic Fluid. in Principles of Regenerative Medicine. Elsevier Inc., pp. 223-239. https://doi.org/10.1016/B978-0-12-381422-7.10012-4
Cananzi M, Atala A, Coppi PD. Stem Cells from Amniotic Fluid. In Principles of Regenerative Medicine. Elsevier Inc. 2011. p. 223-239 https://doi.org/10.1016/B978-0-12-381422-7.10012-4
Cananzi, Mara ; Atala, Anthony ; Coppi, Paolo de. / Stem Cells from Amniotic Fluid. Principles of Regenerative Medicine. Elsevier Inc., 2011. pp. 223-239
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