Stem cell mobilization by hyperbaric oxygen

Stephen R. Thom, Veena M. Bhopale, Omaida C. Velazquez, Lee J. Goldstein, Lynne H. Thom, Donald G. Buerk

Research output: Contribution to journalArticle

194 Scopus citations

Abstract

We hypothesized that exposure to hyperbaric oxygen (HBO2) would mobilize stem/progenitor cells from the bone marrow by a nitric oxide (·NO) -dependent mechanism. The population of CD34+ cells in the peripheral circulation of humans doubled in response to a single exposure to 2.0 atmospheres absolute (ATA) O2 for 2 h. Over a course of 20 treatments, circulating CD34+ cells increased eightfold, although the overall circulating white cell count was not significantly increased. The number of colony-forming cells (CFCs) increased from 16 ± 2 to 26 ± 3 CFCs/100,000 monocytes plated. Elevations in CFCs were entirely due to the CD34+ subpopulation, but increased cell growth only occurred in samples obtained immediately posttreatment. A high proportion of progeny cells express receptors for vascular endothelial growth factor-2 and for stromal-derived growth factor. In mice, HBO2 increased circulating stem cell factor by 50%, increased the number of circulating cells expressing stem cell antigen-1 and CD34 by 3.4-fold, and doubled the number of CFCs. Bone marrow ·NO concentration increased by 1,008 ± 255 nM in association with HBO2. Stem cell mobilization did not occur in knockout mice lacking genes for endothelial ·NO synthase. Moreover, pretreatment of wild-type mice with a ·NO synthase inhibitor prevented the HBO2-induced elevation in stem cell factor and circulating stem cells. We conclude that HBO2 mobilizes stem/progenitor cells by stimulating ·NO synthesis.

Original languageEnglish (US)
Pages (from-to)H1378-H1386
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume290
Issue number4
DOIs
StatePublished - Apr 1 2006
Externally publishedYes

Keywords

  • CD133
  • CD34
  • cKit
  • Colony-forming cells
  • CXCR4
  • Nitric oxide
  • Progenitor cells

ASJC Scopus subject areas

  • Physiology

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