Staphylococcus epidermidis Boosts Innate Immune Response by Activation of Gamma Delta T Cells and Induction of Perforin-2 in Human Skin

Irena Pastar, Katelyn O’Neill, Laura Padula, Cheyanne R. Head, Jamie L. Burgess, Vivien Chen, Denisse Garcia, Olivera Stojadinovic, Suzanne Hower, Gregory V. Plano, Seth R. Thaller, Marjana Tomic-Canic, Natasa Strbo

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Perforin-2 (P-2) is an antimicrobial protein with unique properties to kill intracellular bacteria. Gamma delta (GD) T cells, as the major T cell population in epithelial tissues, play a central role in protective and pathogenic immune responses in the skin. However, the tissue-specific mechanisms that control the innate immune response and the effector functions of GD T cells, especially the cross-talk with commensal organisms, are not very well understood. We hypothesized that the most prevalent skin commensal microorganism, Staphylococcus epidermidis, may play a role in regulating GD T cell-mediated cutaneous responses. We analyzed antimicrobial protein P-2 expression in human skin at a single cell resolution using an amplified fluorescence in situ hybridization approach to detect P-2 mRNA in combination with immunophenotyping. We show that S. epidermidis activates GD T cells and upregulates P-2 in human skin ex vivo in a cell-specific manner. Furthermore, P-2 upregulation following S. epidermidis stimulation correlates with increased ability of skin cells to kill intracellular Staphylococcus aureus. Our findings are the first to reveal that skin commensal bacteria induce P-2 expression, which may be utilized beneficially to modulate host innate immune responses and protect from skin infections.

Original languageEnglish (US)
Article number550946
JournalFrontiers in immunology
Volume11
DOIs
StatePublished - Sep 16 2020
Externally publishedYes

Keywords

  • Staphylococcus epidermidis
  • cytotoxicity
  • gamma delta T cells
  • human skin
  • innate immunity
  • perforin-2/mpeg-1

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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