TY - JOUR
T1 - Staphylococcus aureus Triggers Induction of miR-15B-5P to Diminish DNA Repair and Deregulate Inflammatory Response in Diabetic Foot Ulcers
AU - Ramirez, Horacio A.
AU - Pastar, Irena
AU - Jozic, Ivan
AU - Stojadinovic, Olivera
AU - Stone, Rivka C.
AU - Ojeh, Nkemcho
AU - Gil, Joel
AU - Davis, Stephen C.
AU - Kirsner, Robert S.
AU - Tomic-Canic, Marjana
N1 - Funding Information:
We are very grateful to patients who generously participated in this research. We are also very grateful to the Wound Healing Clinical Research Team for their help and support throughout this project. We are grateful to Gregory V. Plano for the gift of S. aureus USA300 JE2; Ashley M. Rosa, Karen Garzon, and Vivien Chen from the University of Miami Miller School of Medicine for technical assistance; all members of MT-C’s laboratory for helpful criticisms and overall support, and to Barrientos Lab for technical support. MT-C is the guarantor of this work and, as such, had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. This work was funded by National Institutes of Health grants NR013881 (MT-C), DK086364 (MT-C), NR015649 (MT-C), University of Miami Scientific Awards Committee Award SAC 2013-19 (MT-C), and SAC 2013-06 (IP).
Publisher Copyright:
© 2017 The Authors
PY - 2018/5
Y1 - 2018/5
N2 - Diabetic foot ulcers (DFUs) are a debilitating complication of diabetes in which bacterial presence, including the frequent colonizer Staphylococcus aureus, contributes to inhibition of healing. MicroRNAs (miRs) play a role in healing and host response to bacterial pathogens. However, the mechanisms by which miR response to cutaneous S. aureus contributes to DFU pathophysiology are unknown. Here, we show that S. aureus inhibits wound closure and induces miR-15b-5p in acute human and porcine wound models and in chronic DFUs. Transcriptome analyses of DFU tissue showed induction of miR-15b-5p to be critical, regulating many cellular processes, including DNA repair and inflammatory response, by suppressing downstream targets IKBKB, WEE1, FGF2, RAD50, MSH2, and KIT. Using a human wound model, we confirmed that S. aureus-triggered miR-15b-5p induction results in suppression of the inflammatory- and DNA repair-related genes IKBKB and WEE1. Inhibition of DNA repair and accumulation of DNA breaks was functionally confirmed by the presence of the pH2AX within colonized DFUs. We conclude that S. aureus induces miR-15b-5p, subsequently repressing DNA repair and inflammatory response, showing a mechanism of inhibition of healing in DFUs previously unreported, to our knowledge. This underscores a previously unknown role of DNA damage repair in the pathophysiology of DFUs colonized with S. aureus.
AB - Diabetic foot ulcers (DFUs) are a debilitating complication of diabetes in which bacterial presence, including the frequent colonizer Staphylococcus aureus, contributes to inhibition of healing. MicroRNAs (miRs) play a role in healing and host response to bacterial pathogens. However, the mechanisms by which miR response to cutaneous S. aureus contributes to DFU pathophysiology are unknown. Here, we show that S. aureus inhibits wound closure and induces miR-15b-5p in acute human and porcine wound models and in chronic DFUs. Transcriptome analyses of DFU tissue showed induction of miR-15b-5p to be critical, regulating many cellular processes, including DNA repair and inflammatory response, by suppressing downstream targets IKBKB, WEE1, FGF2, RAD50, MSH2, and KIT. Using a human wound model, we confirmed that S. aureus-triggered miR-15b-5p induction results in suppression of the inflammatory- and DNA repair-related genes IKBKB and WEE1. Inhibition of DNA repair and accumulation of DNA breaks was functionally confirmed by the presence of the pH2AX within colonized DFUs. We conclude that S. aureus induces miR-15b-5p, subsequently repressing DNA repair and inflammatory response, showing a mechanism of inhibition of healing in DFUs previously unreported, to our knowledge. This underscores a previously unknown role of DNA damage repair in the pathophysiology of DFUs colonized with S. aureus.
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U2 - 10.1016/j.jid.2017.11.038
DO - 10.1016/j.jid.2017.11.038
M3 - Article
C2 - 29273315
AN - SCOPUS:85044348261
VL - 138
SP - 1187
EP - 1196
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
SN - 0022-202X
IS - 5
ER -