Staphylococcal enterotoxin B activates purified NK cells to secrete IFN-γ but requires T lymphocytes to augment NK cytotoxicity

John A. D'Orazio, George W Burke, Joan Stein-Streilein

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Abstract

Staphylococcal enterotoxin B (SEB) is known for its effects on T lymphocytes; the mechanism of this activation is well studied. Although it is anticipated that there would be effects on other cells in the milieu, the mechanisms of activation of non-T immune cells are not well understood. This report examines the effects of SEB on human NK cells. Incubation of PBMC with SEB augmented NK cytotoxicity against the NK-sensitive targets K562 and Molt-4 and induced activity against the NK-resistant targets, Mel-30 and Raji. The degree of superantigen-augmented. killer (SAK) activity induced by the SEB treatment correlated with the concentration of the superantigen in the mixture. Specific neutralizing Abs for lymphokines or monokines added to cultures showed that IL-2, IFN-γ, or IL-12 each participated in the phenomenon; addition of all Abs together blocked most, but not all, SAK activity. Highly purified T lymphocytes (but not monocytes) supported the development of SAK activity in enriched cultures of CD56+ cells. The SAK activity was blocked by specific Abs to IL-2 and IFN-γ in cocultures of T cells and CD56+ cells. In addition, SEB induced IFN-γ secretion from sorted CD56+ cells. These data show for the first time that superantigen directly initiated cytokine synthesis in NK cells, and that SAK activity was induced as a consequence of products secreted from activated T lymphocytes. Such a superantigen effect on NK cells would place the NK lymphocyte in a pivotal role in determining the outcome of infection or immunopathology caused by superantigen exposure.

Original languageEnglish
Pages (from-to)1014-1023
Number of pages10
JournalJournal of Immunology
Volume154
Issue number3
StatePublished - Dec 1 1995

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Superantigens
Natural Killer Cells
T-Lymphocytes
Interleukin-2
Monokines
Lymphokines
Interleukin-12
Coculture Techniques
Monocytes
Cell Culture Techniques
staphylococcal enterotoxin B
Lymphocytes
Cytokines
Infection

ASJC Scopus subject areas

  • Immunology

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Staphylococcal enterotoxin B activates purified NK cells to secrete IFN-γ but requires T lymphocytes to augment NK cytotoxicity. / D'Orazio, John A.; Burke, George W; Stein-Streilein, Joan.

In: Journal of Immunology, Vol. 154, No. 3, 01.12.1995, p. 1014-1023.

Research output: Contribution to journalArticle

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