Standardization and further development of antigen retrieval immunohistochemistry: Strategies and future goals

Shan Rong Shi; Richard J. Cote; Clive R. Taylor

Standardization and further development of antigen retrieval immunohistochemistry: Strategies and future goals

Shan Rong Shi, Richard J. Cote, Clive R. Taylor

Research output: Contribution to journal › Review article › peer-review

Abstract

The antigen retrieval (AR) technique has proven to be a simple, effective, and reliable method for routine immunohistochemistry (IHC) based upon worldwide application for nearly a decade. This review focuses on several critical issues that may improve standardization of routine IHC and provides strategies for further development of the AR technique, eg, 'test battery' approach, re-evaluation of clinical significance, technical tips, and future goals. One strategy for further study of the AR technique is based on a hypothesis for the detection of molecular alteration of protein resulting from chemical modification, through which it may be possible to attain a greater understanding of the mechanisms of AR-IHC, thereby contributing to a new field of molecular morphology.

Original language	English (US)
Pages (from-to)	177-192
Number of pages	16
Journal	Journal of Histotechnology
Volume	22
Issue number	3
State	Published - Sep 1 1999
Externally published	Yes

Keywords

Chemical modification
Protein conformation
Test battery

ASJC Scopus subject areas

Cell Biology
Anatomy

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abstract = "The antigen retrieval (AR) technique has proven to be a simple, effective, and reliable method for routine immunohistochemistry (IHC) based upon worldwide application for nearly a decade. This review focuses on several critical issues that may improve standardization of routine IHC and provides strategies for further development of the AR technique, eg, 'test battery' approach, re-evaluation of clinical significance, technical tips, and future goals. One strategy for further study of the AR technique is based on a hypothesis for the detection of molecular alteration of protein resulting from chemical modification, through which it may be possible to attain a greater understanding of the mechanisms of AR-IHC, thereby contributing to a new field of molecular morphology.",

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AU - Cote, Richard J.

AU - Taylor, Clive R.

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N2 - The antigen retrieval (AR) technique has proven to be a simple, effective, and reliable method for routine immunohistochemistry (IHC) based upon worldwide application for nearly a decade. This review focuses on several critical issues that may improve standardization of routine IHC and provides strategies for further development of the AR technique, eg, 'test battery' approach, re-evaluation of clinical significance, technical tips, and future goals. One strategy for further study of the AR technique is based on a hypothesis for the detection of molecular alteration of protein resulting from chemical modification, through which it may be possible to attain a greater understanding of the mechanisms of AR-IHC, thereby contributing to a new field of molecular morphology.

AB - The antigen retrieval (AR) technique has proven to be a simple, effective, and reliable method for routine immunohistochemistry (IHC) based upon worldwide application for nearly a decade. This review focuses on several critical issues that may improve standardization of routine IHC and provides strategies for further development of the AR technique, eg, 'test battery' approach, re-evaluation of clinical significance, technical tips, and future goals. One strategy for further study of the AR technique is based on a hypothesis for the detection of molecular alteration of protein resulting from chemical modification, through which it may be possible to attain a greater understanding of the mechanisms of AR-IHC, thereby contributing to a new field of molecular morphology.

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KW - Protein conformation

KW - Test battery

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M3 - Review article

AN - SCOPUS:0032874184

VL - 22

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JO - Journal of Histotechnology

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SN - 0147-8885

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ER -

Standardization and further development of antigen retrieval immunohistochemistry: Strategies and future goals

Abstract

Keywords

ASJC Scopus subject areas

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