Abstract
The antigen retrieval (AR) technique has proven to be a simple, effective, and reliable method for routine immunohistochemistry (IHC) based upon worldwide application for nearly a decade. This review focuses on several critical issues that may improve standardization of routine IHC and provides strategies for further development of the AR technique, eg, 'test battery' approach, re-evaluation of clinical significance, technical tips, and future goals. One strategy for further study of the AR technique is based on a hypothesis for the detection of molecular alteration of protein resulting from chemical modification, through which it may be possible to attain a greater understanding of the mechanisms of AR-IHC, thereby contributing to a new field of molecular morphology.
Original language | English (US) |
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Pages (from-to) | 177-192 |
Number of pages | 16 |
Journal | Journal of Histotechnology |
Volume | 22 |
Issue number | 3 |
State | Published - Sep 1 1999 |
Externally published | Yes |
Keywords
- Chemical modification
- Protein conformation
- Test battery
ASJC Scopus subject areas
- Cell Biology
- Anatomy