Abstract
The antigen retrieval (AR) technique has proven to be a simple, effective, and reliable method for routine immunohistochemistry (IHC) based upon worldwide application for nearly a decade. This review focuses on several critical issues that may improve standardization of routine IHC and provides strategies for further development of the AR technique, eg, 'test battery' approach, re-evaluation of clinical significance, technical tips, and future goals. One strategy for further study of the AR technique is based on a hypothesis for the detection of molecular alteration of protein resulting from chemical modification, through which it may be possible to attain a greater understanding of the mechanisms of AR-IHC, thereby contributing to a new field of molecular morphology.
| Original language | English |
|---|---|
| Pages (from-to) | 177-192 |
| Number of pages | 16 |
| Journal | Journal of Histotechnology |
| Volume | 22 |
| Issue number | 3 |
| State | Published - Sep 1 1999 |
| Externally published | Yes |
Fingerprint
Keywords
- Chemical modification
- Protein conformation
- Test battery
ASJC Scopus subject areas
- Cell Biology
- Anatomy
Cite this
Standardization and further development of antigen retrieval immunohistochemistry : Strategies and future goals. / Shi, Shan Rong; Cote, Richard J; Taylor, Clive R.
In: Journal of Histotechnology, Vol. 22, No. 3, 01.09.1999, p. 177-192.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Standardization and further development of antigen retrieval immunohistochemistry
T2 - Strategies and future goals
AU - Shi, Shan Rong
AU - Cote, Richard J
AU - Taylor, Clive R.
PY - 1999/9/1
Y1 - 1999/9/1
N2 - The antigen retrieval (AR) technique has proven to be a simple, effective, and reliable method for routine immunohistochemistry (IHC) based upon worldwide application for nearly a decade. This review focuses on several critical issues that may improve standardization of routine IHC and provides strategies for further development of the AR technique, eg, 'test battery' approach, re-evaluation of clinical significance, technical tips, and future goals. One strategy for further study of the AR technique is based on a hypothesis for the detection of molecular alteration of protein resulting from chemical modification, through which it may be possible to attain a greater understanding of the mechanisms of AR-IHC, thereby contributing to a new field of molecular morphology.
AB - The antigen retrieval (AR) technique has proven to be a simple, effective, and reliable method for routine immunohistochemistry (IHC) based upon worldwide application for nearly a decade. This review focuses on several critical issues that may improve standardization of routine IHC and provides strategies for further development of the AR technique, eg, 'test battery' approach, re-evaluation of clinical significance, technical tips, and future goals. One strategy for further study of the AR technique is based on a hypothesis for the detection of molecular alteration of protein resulting from chemical modification, through which it may be possible to attain a greater understanding of the mechanisms of AR-IHC, thereby contributing to a new field of molecular morphology.
KW - Chemical modification
KW - Protein conformation
KW - Test battery
UR - http://www.scopus.com/inward/record.url?scp=0032874184&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032874184&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0032874184
VL - 22
SP - 177
EP - 192
JO - Journal of Histotechnology
JF - Journal of Histotechnology
SN - 0147-8885
IS - 3
ER -