Standardization and further development of antigen retrieval immunohistochemistry: Strategies and future goals

Shan Rong Shi; Richard J. Cote; Clive R. Taylor

Standardization and further development of antigen retrieval immunohistochemistry: Strategies and future goals

Shan Rong Shi, Richard J. Cote, Clive R. Taylor

Research output: Contribution to journal › Review article

Abstract

The antigen retrieval (AR) technique has proven to be a simple, effective, and reliable method for routine immunohistochemistry (IHC) based upon worldwide application for nearly a decade. This review focuses on several critical issues that may improve standardization of routine IHC and provides strategies for further development of the AR technique, eg, 'test battery' approach, re-evaluation of clinical significance, technical tips, and future goals. One strategy for further study of the AR technique is based on a hypothesis for the detection of molecular alteration of protein resulting from chemical modification, through which it may be possible to attain a greater understanding of the mechanisms of AR-IHC, thereby contributing to a new field of molecular morphology.

Original language	English (US)
Pages (from-to)	177-192
Number of pages	16
Journal	Journal of Histotechnology
Volume	22
Issue number	3
State	Published - Sep 1 1999
Externally published	Yes

Keywords

Chemical modification
Protein conformation
Test battery

ASJC Scopus subject areas

Cell Biology
Anatomy

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Shi, S. R., Cote, R. J., & Taylor, C. R. (1999). Standardization and further development of antigen retrieval immunohistochemistry: Strategies and future goals. Journal of Histotechnology, 22(3), 177-192.

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AB - The antigen retrieval (AR) technique has proven to be a simple, effective, and reliable method for routine immunohistochemistry (IHC) based upon worldwide application for nearly a decade. This review focuses on several critical issues that may improve standardization of routine IHC and provides strategies for further development of the AR technique, eg, 'test battery' approach, re-evaluation of clinical significance, technical tips, and future goals. One strategy for further study of the AR technique is based on a hypothesis for the detection of molecular alteration of protein resulting from chemical modification, through which it may be possible to attain a greater understanding of the mechanisms of AR-IHC, thereby contributing to a new field of molecular morphology.

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