Stable in vivo imaging of densely populated glia, axons and blood vessels in the mouse spinal cord using two-photon microscopy

Dimitrios Davalos, Jae K. Lee, W. Bryan Smith, Brendan Brinkman, Mark H. Ellisman, Binhai Zheng, Katerina Akassoglou

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

In vivo imaging has revolutionized our understanding of biological processes in brain physiology and pathology. However, breathing-induced movement artifacts have impeded the application of this powerful tool in studies of the living spinal cord. Here we describe in detail a method to image stably and repetitively, using two-photon microscopy, the living spinal tissue in mice with dense fluorescent cells or axons, without the need for animal intubation or image post-processing. This simplified technique can greatly expand the application of in vivo imaging to study spinal cord injury, regeneration, physiology and disease.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalJournal of Neuroscience Methods
Volume169
Issue number1
DOIs
StatePublished - Mar 30 2008
Externally publishedYes

Keywords

  • Axons
  • In vivo imaging
  • Microglia
  • Spinal cord
  • Two-photon microscopy

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Stable in vivo imaging of densely populated glia, axons and blood vessels in the mouse spinal cord using two-photon microscopy'. Together they form a unique fingerprint.

  • Cite this